Detailed information NTG0348955

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C3377_RS07580
• Genome accession: NZ_CP036450
• Coordinates: 1247447..1248247 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KPNIH45
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1242447..1253247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C3377_RS07560 (C3377_007600)	dksA	1243027..1243482 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
C3377_RS07565 (C3377_007605)	sfsA	1243645..1244361 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
C3377_RS07570 (C3377_007610)	thpR	1244361..1244897 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
C3377_RS07575 (C3377_007615)	hrpB	1244970..1247399 (+)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
C3377_RS07580 (C3377_007620)	pilD	1247447..1248247 (-)	801	WP_002888947.1	prepilin peptidase	Machinery gene
C3377_RS07585 (C3377_007625)	-	1248244..1248990 (-)	747	WP_004145897.1	type II secretion system protein N	-
C3377_RS07590 (C3377_007630)	-	1248980..1249465 (-)	486	WP_004151943.1	type II secretion system protein M	-
C3377_RS07595 (C3377_007635)	gspL	1249458..1250651 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
C3377_RS07600 (C3377_007640)	gspK	1250638..1251624 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
C3377_RS07605 (C3377_007645)	gspJ	1251621..1252217 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
C3377_RS07610 (C3377_007650)	gspI	1252214..1252579 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
C3377_RS07615 (C3377_007655)	gspH	1252576..1253091 (-)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=348955 C3377_RS07580 WP_002888947.1 1247447..1248247(-) (pilD) [Klebsiella pneumoniae strain KPNIH45]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=348955 C3377_RS07580 WP_002888947.1 1247447..1248247(-) (pilD) [Klebsiella pneumoniae strain KPNIH45]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425