Detailed information NTG0348930

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: WN05_RS05355
• Genome accession: NZ_CP036442
• Coordinates: 1074072..1074872 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain ABFPV
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1069072..1079872

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WN05_RS05335 (WN05_05340)	dksA	1069652..1070107 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
WN05_RS05340 (WN05_05345)	sfsA	1070270..1070986 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
WN05_RS05345 (WN05_05350)	thpR	1070986..1071522 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
WN05_RS05350 (WN05_05355)	hrpB	1071595..1074024 (+)	2430	WP_020317204.1	ATP-dependent helicase HrpB	-
WN05_RS05355 (WN05_05360)	pilD	1074072..1074872 (-)	801	WP_002888947.1	prepilin peptidase	Machinery gene
WN05_RS05360 (WN05_05365)	-	1074869..1075615 (-)	747	WP_004145897.1	type II secretion system protein N	-
WN05_RS05365 (WN05_05370)	-	1075605..1076090 (-)	486	WP_004151943.1	type II secretion system protein M	-
WN05_RS05370 (WN05_05375)	gspL	1076083..1077276 (-)	1194	WP_004191957.1	type II secretion system protein GspL	-
WN05_RS05375 (WN05_05380)	gspK	1077263..1078249 (-)	987	WP_004222697.1	type II secretion system minor pseudopilin GspK	-
WN05_RS05380 (WN05_05385)	gspJ	1078246..1078842 (-)	597	WP_004222700.1	type II secretion system minor pseudopilin GspJ	-
WN05_RS05385 (WN05_05390)	gspI	1078839..1079204 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
WN05_RS05390 (WN05_05395)	gspH	1079201..1079716 (-)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=348930 WN05_RS05355 WP_002888947.1 1074072..1074872(-) (pilD) [Klebsiella pneumoniae strain ABFPV]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=348930 WN05_RS05355 WP_002888947.1 1074072..1074872(-) (pilD) [Klebsiella pneumoniae strain ABFPV]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425