Detailed information NTG0348921

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: WN11_RS14790
• Genome accession: NZ_CP036438
• Coordinates: 2987275..2988075 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain ABFQB
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2982275..2993075

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WN11_RS14770 (WN11_14810)	dksA	2982855..2983310 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
WN11_RS14775 (WN11_14815)	sfsA	2983473..2984189 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
WN11_RS14780 (WN11_14820)	thpR	2984189..2984725 (-)	537	WP_131138755.1	RNA 2',3'-cyclic phosphodiesterase	-
WN11_RS14785 (WN11_14825)	hrpB	2984798..2987227 (+)	2430	WP_004894175.1	ATP-dependent helicase HrpB	-
WN11_RS14790 (WN11_14830)	pilD	2987275..2988075 (-)	801	WP_002888947.1	prepilin peptidase	Machinery gene
WN11_RS14795 (WN11_14835)	-	2988072..2988818 (-)	747	WP_004145897.1	type II secretion system protein N	-
WN11_RS14800 (WN11_14840)	-	2988808..2989293 (-)	486	WP_004183037.1	type II secretion system protein M	-
WN11_RS14805 (WN11_14845)	gspL	2989286..2990479 (-)	1194	WP_004894169.1	type II secretion system protein GspL	-
WN11_RS14810 (WN11_14850)	gspK	2990466..2991452 (-)	987	WP_004894165.1	type II secretion system minor pseudopilin GspK	-
WN11_RS14815 (WN11_14855)	gspJ	2991449..2992045 (-)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
WN11_RS14820 (WN11_14860)	gspI	2992042..2992407 (-)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
WN11_RS14825 (WN11_14865)	gspH	2992404..2992919 (-)	516	WP_004194939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=348921 WN11_RS14790 WP_002888947.1 2987275..2988075(-) (pilD) [Klebsiella pneumoniae strain ABFQB]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=348921 WN11_RS14790 WP_002888947.1 2987275..2988075(-) (pilD) [Klebsiella pneumoniae strain ABFQB]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425