Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GIJ02_RS23075 Genome accession   NZ_CP045827
Coordinates   4552219..4552659 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain AUSMDU00014361     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4547219..4557659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIJ02_RS23055 (GIJ02_23040) aroP 4548217..4549590 (+) 1374 WP_012817732.1 aromatic amino acid transporter AroP -
  GIJ02_RS23060 (GIJ02_23045) ampE 4549633..4550487 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  GIJ02_RS23065 (GIJ02_23050) ampD 4550484..4551035 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GIJ02_RS23070 (GIJ02_23055) nadC 4551123..4552016 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GIJ02_RS23075 (GIJ02_23060) pilA 4552219..4552659 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  GIJ02_RS23080 (GIJ02_23065) pilB 4552669..4554054 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  GIJ02_RS23085 (GIJ02_23070) hofC 4554044..4555246 (+) 1203 WP_000157234.1 protein transport protein HofC -
  GIJ02_RS23090 (GIJ02_23075) guaC 4555281..4556324 (-) 1044 WP_001217338.1 GMP reductase -
  GIJ02_RS29235 - 4556480..4556524 (-) 45 WP_120795372.1 protein YacM -
  GIJ02_RS23095 (GIJ02_23080) coaE 4556549..4557169 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=348164 GIJ02_RS23075 WP_000360902.1 4552219..4552659(+) (pilA) [Escherichia coli strain AUSMDU00014361]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=348164 GIJ02_RS23075 WP_000360902.1 4552219..4552659(+) (pilA) [Escherichia coli strain AUSMDU00014361]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377