Detailed information NTG0348011

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CK244_RS06025
• Genome accession: NZ_CP036320
• Coordinates: 1201112..1201912 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain VBA2172
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1196112..1206912

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CK244_RS05990 (CK244_005990)	gspH	1196268..1196783 (+)	516	WP_131082189.1	type II secretion system minor pseudopilin GspH	-
CK244_RS05995 (CK244_005995)	gspI	1196780..1197145 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
CK244_RS06000 (CK244_006000)	gspJ	1197142..1197738 (+)	597	WP_064162459.1	type II secretion system minor pseudopilin GspJ	-
CK244_RS06005 (CK244_006005)	gspK	1197735..1198721 (+)	987	WP_077265688.1	type II secretion system minor pseudopilin GspK	-
CK244_RS06010 (CK244_006010)	gspL	1198708..1199901 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
CK244_RS06015 (CK244_006015)	-	1199894..1200379 (+)	486	WP_004145896.1	type II secretion system protein M	-
CK244_RS06020 (CK244_006020)	-	1200369..1201115 (+)	747	WP_023282189.1	type II secretion system protein N	-
CK244_RS06025 (CK244_006025)	pilD	1201112..1201912 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
CK244_RS06030 (CK244_006030)	hrpB	1201960..1204389 (-)	2430	WP_064162458.1	ATP-dependent helicase HrpB	-
CK244_RS06035 (CK244_006035)	thpR	1204462..1204998 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
CK244_RS06040 (CK244_006040)	sfsA	1204998..1205714 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
CK244_RS06045 (CK244_006045)	dksA	1205877..1206332 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=348011 CK244_RS06025 WP_004191960.1 1201112..1201912(+) (pilD) [Klebsiella pneumoniae strain VBA2172]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=348011 CK244_RS06025 WP_004191960.1 1201112..1201912(+) (pilD) [Klebsiella pneumoniae strain VBA2172]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421