Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXZ61_RS16865 Genome accession   NZ_CP036282
Coordinates   3639529..3640641 (+) Length   370 a.a.
NCBI ID   WP_142812861.1    Uniprot ID   -
Organism   Rhodoferax aquaticus strain Gr-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3634529..3645641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXZ61_RS16850 (EXZ61_16860) hemL 3635308..3636612 (+) 1305 WP_142812858.1 glutamate-1-semialdehyde 2,1-aminomutase -
  EXZ61_RS16855 (EXZ61_16865) - 3636609..3637916 (-) 1308 WP_237219194.1 pitrilysin family protein -
  EXZ61_RS16860 (EXZ61_16870) - 3638006..3639499 (-) 1494 WP_142812860.1 pitrilysin family protein -
  EXZ61_RS16865 (EXZ61_16875) pilA 3639529..3640641 (+) 1113 WP_142812861.1 signal recognition particle-docking protein FtsY Machinery gene
  EXZ61_RS16870 (EXZ61_16880) - 3640729..3641928 (+) 1200 WP_142812862.1 ABC transporter substrate-binding protein -
  EXZ61_RS16875 (EXZ61_16885) - 3641992..3643023 (+) 1032 WP_142812863.1 GGDEF domain-containing protein -
  EXZ61_RS16880 (EXZ61_16890) - 3643208..3643891 (+) 684 WP_142812864.1 dienelactone hydrolase family protein -
  EXZ61_RS16885 (EXZ61_16895) - 3643881..3645182 (+) 1302 WP_142812865.1 Hsp70 family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 38321.31 Da        Isoelectric Point: 6.8924

>NTDB_id=347857 EXZ61_RS16865 WP_142812861.1 3639529..3640641(+) (pilA) [Rhodoferax aquaticus strain Gr-4]
MFSFFKKKSPPPPAAAPVAAPVAAPAELPSAAPAAAFSAASAAASPALGGSLIGSALVTPIDIPVPSATPPERQKWLDKL
KAGLGKTASNISGVFVGAQIDEALYEDLEGALLMSDAGVPATEYVLKELRRKVKDSGITHPVALKNVLTATLTEMLQPLQ
KPLVIGAQTPTVIMVAGVNGAGKTTSIGKLTKHLADSQATVLLAAADTFRAAAREQLTVWADRNTVEIISQDGADPAAVS
FDAVSAGKARGKDVVLIDTAGRLPTQLHLMEELKKIKRVIQKADGTAPHEVLLVIDGNTGQNAVTQVKAFDDALGLTGLI
ITKLDGTAKGGVLAAIARERPIPVYFIGVGEKLDELETFNAREFAQALLA

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=347857 EXZ61_RS16865 WP_142812861.1 3639529..3640641(+) (pilA) [Rhodoferax aquaticus strain Gr-4]
ATGTTCAGTTTTTTCAAAAAAAAATCCCCTCCCCCACCGGCTGCAGCGCCCGTTGCAGCACCTGTTGCAGCACCCGCTGA
GCTGCCTAGCGCTGCACCGGCAGCCGCATTCAGCGCCGCATCAGCGGCTGCCAGCCCCGCCTTGGGCGGCTCCCTCATTG
GCAGTGCGCTGGTCACGCCCATCGACATTCCCGTTCCCAGTGCCACACCGCCTGAACGCCAAAAGTGGCTGGACAAGCTC
AAGGCGGGCCTGGGCAAAACAGCGTCCAACATCTCGGGCGTGTTTGTAGGCGCCCAGATTGATGAGGCCTTGTATGAAGA
CCTAGAAGGCGCACTGCTCATGTCAGACGCAGGGGTACCCGCCACAGAATACGTGCTCAAAGAGTTGCGGCGCAAAGTCA
AAGACAGCGGTATTACCCACCCCGTCGCCCTGAAAAACGTGCTGACCGCCACCTTGACCGAGATGCTGCAGCCCTTGCAA
AAGCCCTTGGTCATTGGCGCGCAAACGCCCACCGTCATCATGGTGGCCGGAGTCAACGGCGCGGGCAAGACCACCAGCAT
TGGCAAACTCACCAAGCATTTGGCAGACAGCCAAGCCACTGTGCTGCTGGCAGCGGCCGACACTTTCCGCGCGGCAGCAC
GTGAGCAGCTCACCGTGTGGGCCGACCGCAACACGGTCGAAATCATCAGCCAAGACGGTGCCGACCCCGCTGCCGTGAGC
TTTGACGCAGTCAGCGCTGGCAAAGCACGCGGTAAAGACGTGGTGCTGATTGACACCGCTGGTCGCCTGCCCACGCAGCT
GCACCTGATGGAAGAGCTCAAAAAGATCAAACGCGTGATCCAAAAAGCCGACGGCACCGCCCCCCACGAGGTGCTGCTGG
TCATTGACGGCAACACCGGACAAAACGCTGTCACCCAAGTCAAAGCCTTTGACGACGCCTTAGGCCTCACCGGCCTGATC
ATCACCAAGCTCGACGGAACCGCCAAAGGCGGCGTGCTGGCCGCCATTGCCCGCGAGCGGCCCATCCCGGTGTACTTCAT
TGGCGTGGGCGAGAAGCTCGACGAGTTGGAAACCTTCAACGCACGCGAGTTTGCACAGGCCTTGCTGGCGTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(91-149)

SRP54-type protein, GTPase domain (SRP54)

(170-369)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

55.587

94.324

0.524

Multiple sequence alignment