Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EYC54_RS05845 Genome accession   NZ_CP036252
Coordinates   1197788..1199521 (+) Length   577 a.a.
NCBI ID   WP_053502736.1    Uniprot ID   -
Organism   Xanthomonas oryzae strain NJ611     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1192788..1204521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYC54_RS05825 (EYC54_05825) pilA 1193468..1193881 (+) 414 WP_131077924.1 pilin Machinery gene
  EYC54_RS05830 (EYC54_05830) - 1193953..1195611 (+) 1659 WP_242629559.1 phosphoethanolamine transferase -
  EYC54_RS05835 (EYC54_05835) - 1195682..1196257 (+) 576 WP_242629581.1 hypothetical protein -
  EYC54_RS05840 (EYC54_05840) - 1196227..1197753 (+) 1527 WP_131077926.1 hypothetical protein -
  EYC54_RS05845 (EYC54_05845) pilB 1197788..1199521 (+) 1734 WP_053502736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EYC54_RS05850 (EYC54_05850) - 1199530..1200009 (-) 480 WP_131079000.1 Fic family protein -
  EYC54_RS05855 (EYC54_05855) pilR 1200048..1201442 (-) 1395 WP_024711611.1 sigma-54 dependent transcriptional regulator Regulator
  EYC54_RS05860 (EYC54_05860) - 1201773..1203386 (-) 1614 WP_131077927.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62900.00 Da        Isoelectric Point: 5.6097

>NTDB_id=347648 EYC54_RS05845 WP_053502736.1 1197788..1199521(+) (pilB) [Xanthomonas oryzae strain NJ611]
MNSVSTTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKKLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=347648 EYC54_RS05845 WP_053502736.1 1197788..1199521(+) (pilB) [Xanthomonas oryzae strain NJ611]
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAAAAGAAGCTGG
TGTCGGCATCGCAGCTGGCGGCGGCCAATGCAGTGGAATTCGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTAGATGACATCAAGTTCCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATTCGTCGCACATTGGAGCAGTGGCAGGCAAGCAATGCCTCATTTGGATCATCGCTTGGC
AACGATGATGAGGAAATGGGAGATCTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAAGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGATTTCCGTGTCAGTACGTTGCCGACCCTGTTCGGTGAGA
AGGTGGTGCTGCGTATTCTGGACGGTAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAAAAG
CTGTTCCTGGATGCAATTCATAAGCCTTACGGGATGGTGCTGGTGACCGGGCCCACCGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTCCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GATATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCTTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.44

0.556
  pilB Acinetobacter baylyi ADP1

56.338

98.44

0.555
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilB Vibrio cholerae strain A1552

49.74

100

0.497
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio campbellii strain DS40M4

46.087

99.653

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

46.004

97.574

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

91.508

0.36

Multiple sequence alignment