Detailed information NTG0347454

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EX353_RS12620
• Genome accession: NZ_CP036190
• Coordinates: 2531837..2532637 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain BA34918
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2526837..2537637

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EX353_RS12585 (EX353_12585)	gspH	2526993..2527508 (+)	516	WP_004183045.1	type II secretion system minor pseudopilin GspH	-
EX353_RS12590 (EX353_12590)	gspI	2527505..2527870 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
EX353_RS12595 (EX353_12595)	gspJ	2527867..2528463 (+)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
EX353_RS12600 (EX353_12600)	gspK	2528460..2529446 (+)	987	WP_077250502.1	type II secretion system minor pseudopilin GspK	-
EX353_RS12605 (EX353_12605)	gspL	2529433..2530626 (+)	1194	WP_130627869.1	type II secretion system protein GspL	-
EX353_RS12610 (EX353_12610)	-	2530619..2531104 (+)	486	WP_012737411.1	type II secretion system protein M	-
EX353_RS12615 (EX353_12615)	-	2531094..2531840 (+)	747	WP_012737410.1	type II secretion system protein N	-
EX353_RS12620 (EX353_12620)	pilD	2531837..2532637 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
EX353_RS12625 (EX353_12625)	hrpB	2532685..2535114 (-)	2430	WP_130627870.1	ATP-dependent helicase HrpB	-
EX353_RS12630 (EX353_12630)	thpR	2535187..2535723 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
EX353_RS12635 (EX353_12635)	sfsA	2535723..2536439 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
EX353_RS12640 (EX353_12640)	dksA	2536602..2537057 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=347454 EX353_RS12620 WP_004145898.1 2531837..2532637(+) (pilD) [Klebsiella pneumoniae strain BA34918]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=347454 EX353_RS12620 WP_004145898.1 2531837..2532637(+) (pilD) [Klebsiella pneumoniae strain BA34918]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425