Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   GII83_RS07355 Genome accession   NZ_CP045818
Coordinates   1406936..1409035 (-) Length   699 a.a.
NCBI ID   WP_029726991.1    Uniprot ID   -
Organism   Bacillus subtilis strain MB9_B6     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1401936..1414035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII83_RS07325 (GII83_07325) spo0E 1401991..1402248 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  GII83_RS07330 (GII83_07330) - 1402334..1402765 (+) 432 WP_029726990.1 hypothetical protein -
  GII83_RS07335 (GII83_07335) kinD 1402793..1404313 (-) 1521 WP_072557174.1 sporulation kinase KinD -
  GII83_RS07340 (GII83_07340) mhqR 1404506..1404943 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  GII83_RS07345 (GII83_07345) motB 1404983..1405768 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  GII83_RS07350 (GII83_07350) motA 1405740..1406552 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  GII83_RS07355 (GII83_07355) clpE 1406936..1409035 (-) 2100 WP_029726991.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  GII83_RS07360 (GII83_07360) - 1409401..1410444 (+) 1044 WP_021479408.1 membrane protein -
  GII83_RS07365 (GII83_07365) queC 1410757..1411416 (+) 660 WP_021479407.1 7-cyano-7-deazaguanine synthase QueC -
  GII83_RS07370 (GII83_07370) queD 1411409..1411858 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  GII83_RS07375 (GII83_07375) queE 1411851..1412582 (+) 732 WP_029317718.1 7-carboxy-7-deazaguanine synthase QueE -
  GII83_RS07380 (GII83_07380) queF 1412600..1413097 (+) 498 WP_003218613.1 preQ(1) synthase -
  GII83_RS07385 (GII83_07385) ykvN 1413604..1413960 (-) 357 WP_014417586.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77788.90 Da        Isoelectric Point: 5.2358

>NTDB_id=346935 GII83_RS07355 WP_029726991.1 1406936..1409035(-) (clpE) [Bacillus subtilis strain MB9_B6]
MRCQHCHQNEATIRLNMQINSIHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKNKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAAKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=346935 GII83_RS07355 WP_029726991.1 1406936..1409035(-) (clpE) [Bacillus subtilis strain MB9_B6]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCAATTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTTGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAAAGCGCAGCAAAACAAAGCGGAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCTGGTCTCATTGACCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTCATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGACTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAAGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAAACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGCAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATCGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAAGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACCGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTGATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTAAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTAAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACTGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)

AAA lid domain (AAA_lid_9)

(253-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.557

100

0.592
  clpE Streptococcus pneumoniae TIGR4

54.924

100

0.567
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpC Bacillus subtilis subsp. subtilis str. 168

53.701

90.844

0.488
  clpC Streptococcus pneumoniae Rx1

45.789

93.419

0.428
  clpC Streptococcus pneumoniae D39

45.789

93.419

0.428
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.687

89.7

0.428
  clpC Streptococcus thermophilus LMD-9

45.038

93.705

0.422
  clpC Streptococcus pneumoniae TIGR4

46.361

90.415

0.419
  clpC Streptococcus thermophilus LMG 18311

44.58

93.705

0.418
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.914

86.695

0.363

Multiple sequence alignment