Detailed information NTG0346867

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: EXW25_RS05690
• Genome accession: NZ_CP036111
• Coordinates: 1114000..1117725 (+)
• Length: 1241 a.a.
• NCBI ID: WP_098054377.1
• Uniprot ID: A0AB73R9R2
• Organism: Bacillus toyonensis strain JAS411
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1109000..1122725

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXW25_RS05675 (EXW25_05755)	-	1109034..1109627 (+)	594	WP_000347508.1	TVP38/TMEM64 family protein	-
EXW25_RS05680 (EXW25_05760)	lepB	1109808..1110371 (+)	564	WP_000751914.1	signal peptidase I	-
EXW25_RS05685 (EXW25_05765)	addB	1110488..1114003 (+)	3516	WP_097953204.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
EXW25_RS05690 (EXW25_05770)	addA	1114000..1117725 (+)	3726	WP_098054377.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
EXW25_RS05695 (EXW25_05775)	-	1117749..1118027 (+)	279	WP_000224035.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
EXW25_RS05700 (EXW25_05780)	gerPF	1118116..1118331 (-)	216	WP_001141566.1	spore germination protein GerPF	-
EXW25_RS05705 (EXW25_05785)	-	1118374..1118760 (-)	387	WP_087970724.1	spore germination protein GerPE	-
EXW25_RS05710 (EXW25_05790)	gerPD	1118776..1118970 (-)	195	WP_001052804.1	spore germination protein GerPD	-
EXW25_RS05715 (EXW25_05795)	gerPC	1118977..1119591 (-)	615	WP_001070753.1	spore germination protein GerPC	-
EXW25_RS05720 (EXW25_05800)	gerPB	1119660..1119866 (-)	207	WP_001012514.1	spore germination protein GerPB	-
EXW25_RS05725 (EXW25_05805)	gerPA	1119881..1120102 (-)	222	WP_001111187.1	spore germination protein GerPA	-
EXW25_RS05730 (EXW25_05810)	-	1120199..1120378 (-)	180	WP_000462852.1	aspartyl-phosphate phosphatase Spo0E family protein	-
EXW25_RS05735 (EXW25_05815)	-	1120626..1121525 (+)	900	WP_176281010.1	fumarylacetoacetate hydrolase family protein	-
EXW25_RS05740 (EXW25_05820)	-	1121560..1121841 (-)	282	WP_000926856.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142711.80 Da
• Isoelectric Point: 4.8190

>NTDB_id=346867 EXW25_RS05690 WP_098054377.1 1114000..1117725(+) (addA) [Bacillus toyonensis strain JAS411]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERMIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAALLGMLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDAVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVKLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENDEVKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=346867 EXW25_RS05690 WP_098054377.1 1114000..1117725(+) (addA) [Bacillus toyonensis strain JAS411]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGACG
TGACATTTTAGTCGCAGCAGCAGCTGGATCGGGAAAAACAGCAGTATTAGTTGAACGTATGATTAAAAAGATTATTAACG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAACGCAGCGGCGCAAGAGATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTACTAATTGATGAGCCAGGATCTCAGCATGTAAGAAAACAGCTGAGCCTATTAAATAAAGC
ATCTATTTCAACGATCCATTCATTTTGTTTACAAGTAATTAGAGGGTATTACTACATGCTTGATGTTGATCCTCGTTTTC
GTATTGCAAATCAAACAGAAAATGAGTTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGCTATACGAGTGACCGTAGTGATGATGACTTACAAAGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAACATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGCCCGGCGCCTCGCGTTGAAACCCTACAAGCGGATGCAGCTTTACT
TGGAATGTTATCATCAGCAGCTCGTGAATCGTGGACAAGTGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGCTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTCGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGTGAACAAAGTGAAAATGATGAAGTGAAGCCATCAGCGGTAGCGCTTCAA
TATCGTAATAAATTTGCAGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AACCAGGCTTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGATCGGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGACGCTGACGCTGAATTAAAACTAGGCGCTAGTTATCCAGAAGGTGAAGATGTAGCGGCTGAATTATTATGCA
TTCAGCAAACGGAAGAAGAAGTACTAGAGGGTGAAGAAGGTACGGAAGTCGAAAAGGCGCAACTGGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGAAAAACGGATAGTATGCGACCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCACTTGCAGCAGTACTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCCCACGGAAAGAAAGGGTCATTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAAGAACAAGAAC
TTCATGAAAAACTAGAATGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTATGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGCGTATTATATGACCGCGCAAGGCAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGGACGGCGAGAGCTCTTGGTGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAGGGACTTGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAGCTTGCAATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCTTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTCGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCTGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTGCCGTTACAAAGTGAACGAAAAGAAGAAGTGTACGATAGGTTAATGTGGAAGTACGGGTATGAGG
AAGCAACCTCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
GACGGCAGTCCATGCCGTTATGCAACATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAACAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTATCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAAGAAGATG
GTATTACGCTAATCGATTTCAAAACAGATACGATTGAAGGTAAATTCCCAGGCGGATTTGAACAAGCAAAACCAATTTTA
GAAGATCGCTATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTGAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.248	100	0.535