Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW25_RS05685 Genome accession   NZ_CP036111
Coordinates   1110488..1114003 (+) Length   1171 a.a.
NCBI ID   WP_097953204.1    Uniprot ID   -
Organism   Bacillus toyonensis strain JAS411     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1105488..1119003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW25_RS05650 (EXW25_05725) - 1105543..1106334 (-) 792 WP_098657050.1 phosphotransferase -
  EXW25_RS05655 (EXW25_05730) - 1106354..1106848 (-) 495 WP_000208489.1 DUF6584 family protein -
  EXW25_RS05660 (EXW25_05735) cspA 1107127..1107330 (+) 204 WP_000301521.1 RNA chaperone/antiterminator CspA -
  EXW25_RS05665 (EXW25_05740) - 1107468..1107653 (-) 186 WP_000391370.1 hypothetical protein -
  EXW25_RS05670 (EXW25_05745) - 1107944..1108525 (+) 582 WP_000444437.1 competence protein ComK -
  EXW25_RS05675 (EXW25_05755) - 1109034..1109627 (+) 594 WP_000347508.1 TVP38/TMEM64 family protein -
  EXW25_RS05680 (EXW25_05760) lepB 1109808..1110371 (+) 564 WP_000751914.1 signal peptidase I -
  EXW25_RS05685 (EXW25_05765) addB 1110488..1114003 (+) 3516 WP_097953204.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW25_RS05690 (EXW25_05770) addA 1114000..1117725 (+) 3726 WP_098054377.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW25_RS05695 (EXW25_05775) - 1117749..1118027 (+) 279 WP_000224035.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW25_RS05700 (EXW25_05780) gerPF 1118116..1118331 (-) 216 WP_001141566.1 spore germination protein GerPF -
  EXW25_RS05705 (EXW25_05785) - 1118374..1118760 (-) 387 WP_087970724.1 spore germination protein GerPE -
  EXW25_RS05710 (EXW25_05790) gerPD 1118776..1118970 (-) 195 WP_001052804.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134625.92 Da        Isoelectric Point: 5.1488

>NTDB_id=346866 EXW25_RS05685 WP_097953204.1 1110488..1114003(+) (addB) [Bacillus toyonensis strain JAS411]
MSLRLVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIEREDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQILYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETHYEARPNEKYYDEASVTISTAANL
RAEVEGVAREIRRLVADETYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEMMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDIEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFVTNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVIGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTTNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVDGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=346866 EXW25_RS05685 WP_097953204.1 1110488..1114003(+) (addB) [Bacillus toyonensis strain JAS411]
ATGTCACTTCGATTGGTGATTGGTAGAGCGGGAAGTGGAAAGAGTACACTTTGTTTACGAGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGCAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGAAA
GGGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCTTGGAAGGTGTTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCTGAGCAAAAGTTATTAGCG
AATAAAGTATATGATTTACAGATATTATATGATGATTTTGAACGTGCTTTAATCGGGAAGTATTTAGATTCAGAAGACTA
TTTACAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAGTATGTAAATGGTGCTGAAATTTATATAGATGGGTTTCATTCAT
TTTCTCCGCAAGAACTTGAAATTGTAAGACAGCTCATGATTTGTGGAGCGAGAGTCACAATTACGTTAACGATAGATGAA
AAAACATTAGCGCAACCAGTGAATGAACTGGATTTATTTTATGAAACAACGTTAACATATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGTTTTCATTCTCCGGCATTAGCTCATT
TAGAAACGCATTATGAAGCGCGTCCGAATGAAAAGTATTATGATGAAGCAAGTGTAACAATTAGTACAGCGGCGAATTTA
CGAGCTGAAGTAGAAGGGGTTGCTCGTGAAATTCGTAGACTTGTCGCGGATGAAACGTATCGTTACCGAGATATTGCAGT
ACTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATTCCACATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCATTAGTAGAGTGTATTCGTTCTGCCTTAGAAATTATTAGCGGGAATTGGCGTTAT
GACGCGGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGGAAAGAAATGATGCGTGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGTT
ACCGTTCACTTGACGATACAAACGGAATGATAACAGATATTGAACGTGAAATGGAAGAGAAAATAAACAGGCTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCGGGAACAGTGATGCAAATGTGCGAAGCGGTTTA
CTTATTTTTAGAAGAACTTGACGTTCCAAAGAAGTTAGAAGAATTACGTATACGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCAACAGACCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAAATGCTTGGTGAGGAGAAG
ATGTCACTATCTATGTTCACGGACGTTATGTCGACAGGACTTGAGGCACTTCAATTCGCTAACATTCCGCCATCATTAGA
TCAAGTATTAGTTGCCAATATTGATCATTCCAGGTTATCAGATGTGAAAGCAACGTTTATTATCGGCGTAAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGAATTGAACTC
GCGCCAACGACGAGACAAACTTTATTAGAAGAGCAGTTCGTTATGTATCAGATGGTAACGAGAGCGTCTGAGAAATTATA
TATTTCATGCCCACTTGCAGATGAAGAAGGAAAGACATTACTTGCATCTAGCTTTATTAAGAAAATAAAAAGAATGTTTC
CTAATGTGAAAGACTCGTTTGTTACGAATGACGTAAACGACTTATCGCGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTGACGTTATCTTATGTCATGCAGCAACTGCAAACGTGGAAGCGATACGGATTTGAAGGGAATCTAGATTTTTGGTG
GGACGTCTATAACTTCTACGTCACTTCGGATGAATGGAAACAAAAAAGTAGTCGCGTGTTATCAAGTTTATTCTATCGAA
ATCGTGCGCAGAAACTAAGTACGGTAGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
CTCTTCAACCGCTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTAGATGC
ACCAGATATCGGTGAACTATTCCATGCAGCACTGAAGAGAATTGCAGACAGGCTATTACGCGAAAACCGCACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAAAGACAAATTTTA
TTAAGCTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
CGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAGGTGCCATTCGGTATGGGCGGTACTGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTTATCGGCCGTATTGACCGTGTGGATAAGGCTGAAGATGAAAACGGA
ACATTCCTTCGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTCGTTACTACAAATGCACAAACGTGGATGAAAAAAGGCCACGCAGCATCACCTGCTGGTG
TACTGTATTTTCATATTCATAACCCGATTGTTGAAATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGCCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCACGTTCTAGCATTGCAAGTGAGCAAGAGTTTA
ACGTGCTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATTGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTCTGTCAGTTTGATGAATCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGACGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.488

100

0.495

Multiple sequence alignment