Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW34_RS25110 Genome accession   NZ_CP036102
Coordinates   4883847..4885196 (-) Length   449 a.a.
NCBI ID   WP_033728259.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS391     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4878847..4890196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW34_RS25085 (EXW34_25575) - 4880388..4881326 (-) 939 Protein_4901 helix-turn-helix transcriptional regulator -
  EXW34_RS25090 (EXW34_25580) - 4881409..4881852 (+) 444 WP_002191161.1 VOC family protein -
  EXW34_RS25095 (EXW34_25585) hpf 4881956..4882498 (-) 543 WP_002034758.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EXW34_RS25100 (EXW34_25590) cspC 4882819..4883016 (-) 198 WP_001990088.1 cold shock protein CspC -
  EXW34_RS25105 (EXW34_25595) - 4883143..4883847 (-) 705 WP_002191162.1 ComF family protein -
  EXW34_RS25110 (EXW34_25600) comFA 4883847..4885196 (-) 1350 WP_033728259.1 ATP-dependent helicase ComFA Machinery gene
  EXW34_RS25115 (EXW34_25605) - 4885327..4886661 (-) 1335 WP_002191164.1 NlpC/P60 family protein -
  EXW34_RS25120 (EXW34_25610) - 4886895..4887209 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  EXW34_RS25125 (EXW34_25615) - 4887372..4888214 (-) 843 WP_002016143.1 DegV family protein -
  EXW34_RS25130 (EXW34_25620) - 4888453..4889088 (+) 636 WP_002191166.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50871.22 Da        Isoelectric Point: 9.5337

>NTDB_id=346849 EXW34_RS25110 WP_033728259.1 4883847..4885196(-) (comFA) [Bacillus mycoides strain JAS391]
MLAGRQLLLEELSSDLQNKLDHLKRNRDVVCVQGVIKKSSKYMCQRCGNIEQRLFVSLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCSAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=346849 EXW34_RS25110 WP_033728259.1 4883847..4885196(-) (comFA) [Bacillus mycoides strain JAS391]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCAGAATAAATTGGATCATCTGAAAAGGAATCG
AGACGTCGTTTGTGTGCAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGTATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGTGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTAGTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTGTTAAAAATTTACAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTTCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.34

98.218

0.494
  comFA Latilactobacillus sakei subsp. sakei 23K

41.206

88.641

0.365

Multiple sequence alignment