Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW34_RS20600 Genome accession   NZ_CP036102
Coordinates   4052302..4053345 (-) Length   347 a.a.
NCBI ID   WP_002014996.1    Uniprot ID   R8I3I9
Organism   Bacillus mycoides strain JAS391     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4047302..4058345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW34_RS20555 (EXW34_20980) - 4047700..4047900 (-) 201 WP_215601388.1 YqzE family protein -
  EXW34_RS20560 (EXW34_20985) - 4047939..4048436 (-) 498 WP_002190663.1 shikimate kinase -
  EXW34_RS20565 (EXW34_20990) - 4048588..4049238 (-) 651 WP_002190664.1 2OG-Fe(II) oxygenase -
  EXW34_RS20570 (EXW34_20995) comGG 4049413..4049784 (-) 372 WP_002129085.1 competence type IV pilus minor pilin ComGG -
  EXW34_RS20575 (EXW34_21000) comGF 4049781..4050242 (-) 462 WP_002190666.1 competence type IV pilus minor pilin ComGF -
  EXW34_RS20580 (EXW34_21005) - 4050226..4050522 (-) 297 WP_002190667.1 hypothetical protein -
  EXW34_RS20585 (EXW34_21010) comGD 4050515..4050970 (-) 456 WP_002088622.1 competence type IV pilus minor pilin ComGD -
  EXW34_RS20590 (EXW34_21015) comGC 4050967..4051266 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  EXW34_RS20595 (EXW34_21020) comGB 4051278..4052315 (-) 1038 WP_002138336.1 competence type IV pilus assembly protein ComGB -
  EXW34_RS20600 (EXW34_21025) comGA 4052302..4053345 (-) 1044 WP_002014996.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW34_RS20605 (EXW34_21030) - 4053551..4054246 (+) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  EXW34_RS20610 (EXW34_21035) - 4054525..4054767 (+) 243 WP_002190671.1 DUF2626 domain-containing protein -
  EXW34_RS20615 (EXW34_21040) - 4054875..4056269 (+) 1395 WP_002138338.1 L-cystine transporter -
  EXW34_RS20620 (EXW34_21045) - 4056350..4056742 (-) 393 WP_002190672.1 hypothetical protein -
  EXW34_RS20625 (EXW34_21050) - 4056830..4057045 (-) 216 WP_002067106.1 DUF3912 family protein -
  EXW34_RS20630 (EXW34_21055) - 4057298..4057609 (+) 312 WP_002129099.1 hypothetical protein -
  EXW34_RS20635 (EXW34_21060) - 4057646..4058134 (-) 489 WP_002190674.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39264.73 Da        Isoelectric Point: 9.3368

>NTDB_id=346838 EXW34_RS20600 WP_002014996.1 4052302..4053345(-) (comGA) [Bacillus mycoides strain JAS391]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346838 EXW34_RS20600 WP_002014996.1 4052302..4053345(-) (comGA) [Bacillus mycoides strain JAS391]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8I3I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363

Multiple sequence alignment