Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW48_RS06030 Genome accession   NZ_CP036101
Coordinates   1218868..1222383 (+) Length   1171 a.a.
NCBI ID   WP_215562846.1    Uniprot ID   -
Organism   Bacillus wiedmannii strain JAS28/2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1213868..1227383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW48_RS05995 (EXW48_06005) cspA 1214353..1214556 (+) 204 WP_000301521.1 RNA chaperone/antiterminator CspA -
  EXW48_RS06000 (EXW48_06010) - 1214670..1214831 (+) 162 Protein_1089 IS5/IS1182 family transposase -
  EXW48_RS06005 (EXW48_06015) - 1214989..1215201 (-) 213 WP_046958331.1 hypothetical protein -
  EXW48_RS06010 (EXW48_06020) - 1215518..1215703 (-) 186 WP_000391437.1 hypothetical protein -
  EXW48_RS06015 (EXW48_06025) - 1215987..1216568 (+) 582 WP_046958330.1 competence protein ComK -
  EXW48_RS28685 (EXW48_06030) - 1216811..1217047 (+) 237 WP_046958329.1 hypothetical protein -
  EXW48_RS06020 (EXW48_06040) - 1217398..1217991 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  EXW48_RS06025 (EXW48_06045) lepB 1218188..1218751 (+) 564 WP_000751908.1 signal peptidase I -
  EXW48_RS06030 (EXW48_06050) addB 1218868..1222383 (+) 3516 WP_215562846.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW48_RS06035 (EXW48_06055) addA 1222380..1226105 (+) 3726 WP_215562847.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW48_RS06040 (EXW48_06060) - 1226118..1226405 (+) 288 WP_001240558.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW48_RS06045 (EXW48_06065) gerPF 1226487..1226702 (-) 216 WP_001141570.1 spore germination protein GerPF -
  EXW48_RS06050 (EXW48_06070) - 1226745..1227131 (-) 387 WP_046958325.1 spore germination protein GerPE -
  EXW48_RS06055 (EXW48_06075) gerPD 1227147..1227341 (-) 195 WP_001052804.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134298.35 Da        Isoelectric Point: 5.1436

>NTDB_id=346767 EXW48_RS06030 WP_215562846.1 1218868..1222383(+) (addB) [Bacillus wiedmannii strain JAS28/2]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRSLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSVAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=346767 EXW48_RS06030 WP_215562846.1 1218868..1222383(+) (addB) [Bacillus wiedmannii strain JAS28/2]
ATGTCACTTCGATTTGTAATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
ACAACGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGTTACA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCAGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTGGATTCAGAAGACTA
CCTACAATTGCTAGTCGAAAAGCTTCCACAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGTGCGCGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTGGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAGCAACCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCCCGTGAAATTCGTAGTCTTGTGGCAGATGAAGACTATCGTTACCGAGATATTGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGAGATGATTACAGACAGTGAACGTGAAATGGAAGAGAGAATAAATCGACTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGGCTGAAGCGAGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
TTTATTTTTAGAAGAGCTAGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTTGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGAG
TTATTCCGGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGATGTTCTTAGTGTTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACCTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACAAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATATGGAGATAAAATAAAAGGAAGCGTATCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGACATTTTCAAATTGGATGC
GCCAGATATCGGGGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCGGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAAAACGGG
ACGTTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACAGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGAGGTACTGCATCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCGATCGTTGAGATGAAGGGTGACGCATCTGAAGCAGAAATTGAGAAAGAGATTTTA
AAGAAATTTAAAATGAAAGGACTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCGAGTATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATCGATATTGCTCCT
TATAAGAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.233

100

0.494

Multiple sequence alignment