Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EXW48_RS06035 Genome accession   NZ_CP036101
Coordinates   1222380..1226105 (+) Length   1241 a.a.
NCBI ID   WP_215562847.1    Uniprot ID   -
Organism   Bacillus wiedmannii strain JAS28/2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1217380..1231105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW48_RS06020 (EXW48_06040) - 1217398..1217991 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  EXW48_RS06025 (EXW48_06045) lepB 1218188..1218751 (+) 564 WP_000751908.1 signal peptidase I -
  EXW48_RS06030 (EXW48_06050) addB 1218868..1222383 (+) 3516 WP_215562846.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW48_RS06035 (EXW48_06055) addA 1222380..1226105 (+) 3726 WP_215562847.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW48_RS06040 (EXW48_06060) - 1226118..1226405 (+) 288 WP_001240558.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW48_RS06045 (EXW48_06065) gerPF 1226487..1226702 (-) 216 WP_001141570.1 spore germination protein GerPF -
  EXW48_RS06050 (EXW48_06070) - 1226745..1227131 (-) 387 WP_046958325.1 spore germination protein GerPE -
  EXW48_RS06055 (EXW48_06075) gerPD 1227147..1227341 (-) 195 WP_001052804.1 spore germination protein GerPD -
  EXW48_RS06060 (EXW48_06080) gerPC 1227348..1227962 (-) 615 WP_001070746.1 spore germination protein GerPC -
  EXW48_RS06065 (EXW48_06085) gerPB 1228031..1228237 (-) 207 WP_001012513.1 spore germination protein GerPB -
  EXW48_RS06070 (EXW48_06090) gerPA 1228252..1228473 (-) 222 WP_001111187.1 spore germination protein GerPA -
  EXW48_RS06075 (EXW48_06095) - 1228568..1228747 (-) 180 WP_000462847.1 aspartyl-phosphate phosphatase Spo0E family protein -
  EXW48_RS06080 (EXW48_06100) - 1228994..1229893 (+) 900 WP_001241717.1 fumarylacetoacetate hydrolase family protein -
  EXW48_RS06085 (EXW48_06105) - 1229929..1230213 (-) 285 WP_215562848.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142780.10 Da        Isoelectric Point: 4.8661

>NTDB_id=346768 EXW48_RS06035 WP_215562847.1 1222380..1226105(+) (addA) [Bacillus wiedmannii strain JAS28/2]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSSAACKSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSESGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYTVYDRKTNEMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSTFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYEYDTNWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKVEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=346768 EXW48_RS06035 WP_215562847.1 1222380..1226105(+) (addA) [Bacillus wiedmannii strain JAS28/2]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAACTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAAGAGTACGGAATTGAA
GATAATAGTATTTTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGCGCCCCGTGTTGAAACACTGCAAGCAGATGCAGTTTTACT
TGGAACGTTGTCATCAGCAGCATGTAAATCGTGGACAAGCGTTTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGTTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGACATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGTGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGCTTACAAATTTTAAGTGAACAAAGTGAAAGTGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGCCTGTTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATTAGACGGCGGAATGAAAATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTGAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACAGAAGAAGAAGTGCAAGACGGAGAAGAAGGTGCGGAAGTAGAAAAAGCACAACTTGAAGCTCGCCTTATG
GCGCAGCGAATAAAGGCTATGGTTGATTCAGGATATACCGTCTATGATCGCAAAACGAATGAAATGCGACAAGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCACCAATCGTTGGGTTAAATGATGAGGAACTTGCGACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCACATTCTTAAAAGGGGCACCACTTGAAGAAGAGCAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
TCGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACTGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGGTGAAATTTATGAATATGACACGAACTGGAAA
GTGGAAGTTGTTGACGGCAAAACGTTACTTGCACCGGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGCTACAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGATTGACGTACGCAGAGCGTGG
GACAGCAGTCCATGCTGTTATGCAACATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAGCAAATTGCCG
GAATGGTAAATAAGGAACTATTAACATTCGAACAAGCAGAAGAAATAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGATGAAACAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGACTTCAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAAGCGAAACCGATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAGGCGCTTGAAAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTCAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1232)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.168

100

0.534

Multiple sequence alignment