Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EXW48_RS00530 Genome accession   NZ_CP036101
Coordinates   97575..98951 (+) Length   458 a.a.
NCBI ID   WP_001085205.1    Uniprot ID   A0A2A8XI58
Organism   Bacillus wiedmannii strain JAS28/2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92575..103951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW48_RS00510 (EXW48_00510) ctsR 92768..93229 (+) 462 WP_001244556.1 transcriptional regulator CtsR -
  EXW48_RS00515 (EXW48_00515) - 93403..93951 (+) 549 WP_000128404.1 UvrB/UvrC motif-containing protein -
  EXW48_RS00520 (EXW48_00520) - 93956..95020 (+) 1065 WP_046958121.1 protein arginine kinase -
  EXW48_RS00525 (EXW48_00525) clpC 95043..97478 (+) 2436 WP_046958120.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  EXW48_RS00530 (EXW48_00530) radA 97575..98951 (+) 1377 WP_001085205.1 DNA repair protein RadA Machinery gene
  EXW48_RS00535 (EXW48_00535) disA 98955..100028 (+) 1074 WP_000392165.1 DNA integrity scanning diadenylate cyclase DisA -
  EXW48_RS00540 (EXW48_00540) - 100191..101294 (+) 1104 WP_000919686.1 PIN/TRAM domain-containing protein -
  EXW48_RS00545 (EXW48_00545) ispD 101311..101991 (+) 681 WP_046958119.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EXW48_RS00550 (EXW48_00550) ispF 102107..102583 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49874.55 Da        Isoelectric Point: 7.4250

>NTDB_id=346762 EXW48_RS00530 WP_001085205.1 97575..98951(+) (radA) [Bacillus wiedmannii strain JAS28/2]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTTPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=346762 EXW48_RS00530 WP_001085205.1 97575..98951(+) (radA) [Bacillus wiedmannii strain JAS28/2]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGCCCTGGTTG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGGCTTAATTATGCCAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAATCTGAGGCACGTATTGAAACGAAATTCCAAGAGTTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAAACTGATCTACAGCGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGAAGTGTAGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATTCCTATTTTTA
TCGTCGGACATGTAACAAAAGAGGGGGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGTCATCATACATATCGTATCTTGCGAGCTGTGAAGAATCGTTTTGGTTCCACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTCTTAAACCCTTCTGAAATTTTTCTTGAGGAAAGACCCGTTGGAGTCGCAG
GATCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTATTGCAAAACCAAGACGCATATTTAAAAGTAGCAGGTGGTTTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATTGCTTCAAGTTTTAGAGATAAATCTACGACACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGGGGATGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCTAATTTAGGGGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A8XI58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment