Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EXW46_RS00965 Genome accession   NZ_CP036085
Coordinates   174784..176610 (-) Length   608 a.a.
NCBI ID   WP_060749443.1    Uniprot ID   A0A109GGW3
Organism   Bacillus mycoides strain JAS12/5     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 169784..181610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW46_RS00930 (EXW46_00930) - 170191..170988 (-) 798 WP_002011365.1 NAD kinase -
  EXW46_RS00935 (EXW46_00935) - 171007..171645 (-) 639 WP_002125951.1 GTP pyrophosphokinase family protein -
  EXW46_RS00940 (EXW46_00940) - 171676..172047 (-) 372 WP_002011362.1 hypothetical protein -
  EXW46_RS00945 (EXW46_00945) - 172192..172770 (+) 579 WP_060749442.1 CYTH domain-containing protein -
  EXW46_RS00950 (EXW46_00950) - 172956..173354 (+) 399 WP_000043388.1 hypothetical protein -
  EXW46_RS00955 (EXW46_00955) - 173354..174247 (+) 894 WP_002125944.1 ClpXP adapter SpxH family protein -
  EXW46_RS00960 - 174477..174650 (+) 174 WP_000748141.1 hypothetical protein -
  EXW46_RS00965 (EXW46_00960) pepF 174784..176610 (-) 1827 WP_060749443.1 oligoendopeptidase F Regulator
  EXW46_RS00970 (EXW46_00965) - 176661..177896 (-) 1236 WP_215576880.1 competence protein CoiA -
  EXW46_RS00975 (EXW46_00970) - 177988..179532 (-) 1545 WP_215576879.1 cardiolipin synthase -
  EXW46_RS00980 (EXW46_00975) mecA 179605..180288 (-) 684 WP_002011351.1 adaptor protein MecA Regulator
  EXW46_RS00985 (EXW46_00980) - 180725..181399 (+) 675 WP_002030376.1 TerC family protein -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70287.68 Da        Isoelectric Point: 4.6710

>NTDB_id=346560 EXW46_RS00965 WP_060749443.1 174784..176610(-) (pepF) [Bacillus mycoides strain JAS12/5]
MSEQNKAKTLPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLQENRELSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREAAFKAVFETYGKFKNTFAST
LSGAVKRNNFNARIRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVQEVKM
NVKYEEAQDLLLKSLNVLGDEYVEILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLYTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDKEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=346560 EXW46_RS00965 WP_060749443.1 174784..176610(-) (pepF) [Bacillus mycoides strain JAS12/5]
ATGTCTGAACAAAATAAAGCAAAAACATTACCAGATCGCAATGAGATTGAAGAAGCAAGTACATGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTGCCAAAGTTAACTGAATTTAAAGGGA
AGCTTGGCGACTCTGCAGACAATTTGCTTGAGGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGCTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCACTGAATGATCGCGCAACAAATTTATA
CTCACAAGTATCTAGTAGCACAGCGTATATCGTACCTGAAATTTTATCTATTTCAGAAGATACATTGCAAACATTCTTAC
AAGAAAATCGAGAACTAAGTGTATATGAGCATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCTGAGGCA
GAGGAAGCGTTATTAGCAGAAGCATCTGAAGTGATGAGTGCATCAAGTAATACATTTGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAGGTAGAAATAACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTTCGCGAAGCTGCATTCAAAGCTGTATTTGAAACGTATGGGAAATTTAAGAACACATTTGCAAGTACG
TTAAGCGGGGCAGTGAAACGTAATAATTTCAATGCACGTATTCGTAAATATGACTCTGCTCGCCAAGCAGCATTAAGCAA
TAATAATATTCCTGAAGCAGTATACGATCAACTCGTTGAAACGGTAAATGACAACTTACATTTATTACACCGTTACATTG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTACGATTTATATACACCACTTGTACAAGAAGTGAAAATG
AATGTGAAGTATGAAGAAGCGCAAGACCTGTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGAAATTTTAAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTACGAGAATAAAGGCAAACGAAGCGGAGCATATTCATCTGGTGCATACG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATATACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTATTACACAAGAAAGACACAGCCACATGTATACGGTGATTATTCAATCTTCGTTGCAGAGGTAGCATCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAAAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACAATGTTTGCAGAGTTCGAGCATATCATTCATAAAAAAGTACAA
GAAGGACATGCGGTTACGCCAGATATGTTAACGGAGATCTACTACGATTTAAATAAGAAATATTTCGGGGATGCTTTAGT
AATCGATAAAGAAATCGGTTTAGAATGGTCTCGTATTCCGCACTTCTATTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACTGCGTTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGTTACATTAACGAATTC
TTAAAAGCGGGAAGCTCCGATTATCCAATTGAAGTGTTGAAAAAAGCAGGAGTTGATATGGCATCTCCTGAACCAGTGAA
AGAGGCACTTCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCGTTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A109GGW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.747

97.533

0.485

Multiple sequence alignment