Detailed information NTG0346421

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: EXW41_RS05660
• Genome accession: NZ_CP036070
• Coordinates: 1099960..1103685 (+)
• Length: 1241 a.a.
• NCBI ID: WP_215559006.1
• Uniprot ID: -
• Organism: Bacillus wiedmannii strain JAS07/5
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1094960..1108685

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXW41_RS05645 (EXW41_05685)	-	1095118..1095711 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
EXW41_RS05650 (EXW41_05690)	lepB	1095768..1096331 (+)	564	WP_048567025.1	signal peptidase I	-
EXW41_RS05655 (EXW41_05695)	addB	1096448..1099963 (+)	3516	WP_088062437.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
EXW41_RS05660 (EXW41_05700)	addA	1099960..1103685 (+)	3726	WP_215559006.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
EXW41_RS05665 (EXW41_05705)	-	1103698..1103985 (+)	288	WP_060489297.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
EXW41_RS05670 (EXW41_05710)	gerPF	1104067..1104282 (-)	216	WP_001141566.1	spore germination protein GerPF	-
EXW41_RS05675 (EXW41_05715)	-	1104325..1104711 (-)	387	WP_000902337.1	spore germination protein GerPE	-
EXW41_RS05680 (EXW41_05720)	gerPD	1104727..1104921 (-)	195	WP_001052804.1	spore germination protein GerPD	-
EXW41_RS05685 (EXW41_05725)	gerPC	1104928..1105542 (-)	615	WP_001070746.1	spore germination protein GerPC	-
EXW41_RS05690 (EXW41_05730)	gerPB	1105611..1105817 (-)	207	WP_001012513.1	spore germination protein GerPB	-
EXW41_RS05695 (EXW41_05735)	gerPA	1105832..1106053 (-)	222	WP_001111187.1	spore germination protein GerPA	-
EXW41_RS05700 (EXW41_05740)	-	1106147..1106326 (-)	180	WP_046958324.1	aspartyl-phosphate phosphatase Spo0E family protein	-
EXW41_RS05705 (EXW41_05745)	-	1106573..1107472 (+)	900	WP_001241717.1	fumarylacetoacetate hydrolase family protein	-
EXW41_RS05710 (EXW41_05750)	-	1107508..1107792 (-)	285	WP_046958323.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142824.02 Da
• Isoelectric Point: 4.8199

>NTDB_id=346421 EXW41_RS05660 WP_215559006.1 1099960..1103685(+) (addA) [Bacillus wiedmannii strain JAS07/5]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAETHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSSAACESWTNVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVTAELLCIQQTEEEVLDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLEWIAPSLYRHRDSEMLLELGQGSIPGEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQINGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=346421 EXW41_RS05660 WP_215559006.1 1099960..1103685(+) (addA) [Bacillus wiedmannii strain JAS07/5]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATATTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCAACGATCCATTCATTTTGTTTACAAGTAATTAGAGGATACTACTATATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAACGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATAGTATTTTCTTTGAACTCGTTGACCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGACACATATT
CGTAAAGCGACTGAACTCGCGATGCTTCCTGACGGTCCGGCGCCCCGCGTTGAAACCCTGCAAGCAGATGCAGTTTTACT
TGGAACGTTGTCATCAGCTGCTTGTGAGTCATGGACAAATGTGTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGTGATAAAGGGATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTCTACAAATTTTAAGTGAACAAAGTGAAAACGGTGAAATGAAGCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGCCTGTTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATTAGACGGCGGAATGAAAATCGATTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGACTATGATGCTGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAACAGCTGAACTACTATGTA
TTCAGCAAACAGAAGAAGAAGTGCTAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCGCAACTTGAAGCTCGCCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCTATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAATGTATTCCGAGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCACCAATAGTTGGACTAAATGATGAAGAACTTGCAACATTGCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAAGAACAAGAAT
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTTTTACTGCATAAAGATTTCGGTTTCGGTTCACAATTTATTGATCCTCGTAAACGTATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATTGGAACAGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGAGTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGGTGAAATTTATGGCTATGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTTGCGCCAGAACCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGAAGAAGTGTATGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGCCCTCGTTTTATGGAGAAAAAAGGATTAACGTACGCTGAAAGAGG
TACGGCAGTTCACGCTGTCATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTAATG
GCATGGTTAATAAAGAATTATTAACATTCGAACAAGCAGAAGAAATAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGACGAAACAATTCTTGTCCAAGGGGTTATTGACTGCATGATTGAAGAGGAAGATG
GTATCACGCTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAGCTTTCTTTATATGCAAAGGCACTTGAAAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAACCATGTTGTAAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.087	100	0.533