Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW41_RS05655 Genome accession   NZ_CP036070
Coordinates   1096448..1099963 (+) Length   1171 a.a.
NCBI ID   WP_088062437.1    Uniprot ID   -
Organism   Bacillus wiedmannii strain JAS07/5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1091448..1104963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW41_RS05625 (EXW41_05660) - 1092142..1092933 (-) 792 WP_060489287.1 phosphotransferase -
  EXW41_RS05630 (EXW41_05665) cspA 1093307..1093510 (+) 204 WP_000301521.1 RNA chaperone/antiterminator CspA -
  EXW41_RS05635 (EXW41_05670) - 1093758..1093943 (-) 186 WP_000391440.1 hypothetical protein -
  EXW41_RS05640 (EXW41_05675) - 1094228..1094809 (+) 582 WP_088062435.1 competence protein ComK -
  EXW41_RS05645 (EXW41_05685) - 1095118..1095711 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  EXW41_RS05650 (EXW41_05690) lepB 1095768..1096331 (+) 564 WP_048567025.1 signal peptidase I -
  EXW41_RS05655 (EXW41_05695) addB 1096448..1099963 (+) 3516 WP_088062437.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW41_RS05660 (EXW41_05700) addA 1099960..1103685 (+) 3726 WP_215559006.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW41_RS05665 (EXW41_05705) - 1103698..1103985 (+) 288 WP_060489297.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW41_RS05670 (EXW41_05710) gerPF 1104067..1104282 (-) 216 WP_001141566.1 spore germination protein GerPF -
  EXW41_RS05675 (EXW41_05715) - 1104325..1104711 (-) 387 WP_000902337.1 spore germination protein GerPE -
  EXW41_RS05680 (EXW41_05720) gerPD 1104727..1104921 (-) 195 WP_001052804.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134349.36 Da        Isoelectric Point: 5.1450

>NTDB_id=346420 EXW41_RS05655 WP_088062437.1 1096448..1099963(+) (addB) [Bacillus wiedmannii strain JAS07/5]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPHSEYVNGAEIYIDGFHSFSPQELEIARQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSVAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=346420 EXW41_RS05655 WP_088062437.1 1096448..1099963(+) (addB) [Bacillus wiedmannii strain JAS07/5]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTGCAAGAAGTTGGT
GGAGCGAGTCGCCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGACGCACATAGTAGCAGTGCAGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTGGATTCAGAAGACTA
CCTACAATTGCTAGTCGAAAAGCTTCCACATTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TTTCACCGCAAGAACTAGAAATTGCAAGACAGCTTATGATTTGCGGTGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTGGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAACCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCGCGTGAAATTCGTAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATATTGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGTGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCCTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGAGATGATTACAGACAGTGAACGTGAAATGGAAGAGAGAATAAATCGACTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGGCTGAAGCGAGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
TTTATTTTTAGAAGAGCTAGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTTTTAT
TTGCGACAGATCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGATGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCATCATTAGA
CCAAGTGTTAATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGAG
TTATTCCGGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGATGTTCTTAGCGTTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACAAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATATGGAGATAAAATAAAAGGAAGCGTATCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGACATTTTCAAACTGGATGC
ACCCGATATCGGGGAACTATTCCATGCAGCGCTGAAGAGAATTGCGGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTAAAGATGGAAGTAGTTGGTCGTATTGACCGTGTTGATAAGGCGGAAGATGAAAATGGG
ACGTTCCTACGTATTATTGATTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACATACGTGGATGAAAAAGGGAGGTACTGCATCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCGATCGTTGAGATGAAGGGTGACGCATCTGAAGCAGAAATTGAGAAAGAGATTTTA
AAGAAATTTAAAATGAAAGGACTTGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAACAAACTTTCGACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGACGGCAGTTTTAGTGCGCGTTCGAGTATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATACGTACACCATACGTTTGAAAATATTGGAAAAGACATTACGGAAGGCGTTATTGATATTGCTCCT
TATAAGAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTCGAAGATAA
CCAGTTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.148

100

0.493

Multiple sequence alignment