Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW62_RS19780 Genome accession   NZ_CP036034
Coordinates   3903236..3904279 (-) Length   347 a.a.
NCBI ID   WP_016106153.1    Uniprot ID   A0A1G4EMB3
Organism   Bacillus mycoides strain BPN57/2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3898236..3909279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW62_RS19735 (EXW62_20185) - 3898640..3898840 (-) 201 WP_002015009.1 YqzE family protein -
  EXW62_RS19740 (EXW62_20190) - 3898879..3899376 (-) 498 WP_098778899.1 shikimate kinase -
  EXW62_RS19745 (EXW62_20200) - 3899523..3900173 (-) 651 WP_215554186.1 2OG-Fe(II) oxygenase -
  EXW62_RS19750 (EXW62_20205) comGG 3900347..3900718 (-) 372 WP_215554187.1 competence type IV pilus minor pilin ComGG -
  EXW62_RS19755 (EXW62_20210) comGF 3900715..3901176 (-) 462 WP_215554188.1 competence type IV pilus minor pilin ComGF -
  EXW62_RS19760 (EXW62_20215) - 3901160..3901456 (-) 297 WP_002160491.1 hypothetical protein -
  EXW62_RS19765 (EXW62_20220) comGD 3901449..3901904 (-) 456 WP_215554189.1 competence type IV pilus minor pilin ComGD -
  EXW62_RS19770 (EXW62_20225) comGC 3901901..3902200 (-) 300 WP_215554190.1 competence type IV pilus major pilin ComGC -
  EXW62_RS19775 (EXW62_20230) comGB 3902212..3903249 (-) 1038 WP_286675778.1 competence type IV pilus assembly protein ComGB -
  EXW62_RS19780 (EXW62_20235) comGA 3903236..3904279 (-) 1044 WP_016106153.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW62_RS19785 (EXW62_20240) - 3904485..3905180 (+) 696 WP_215554192.1 helix-turn-helix domain-containing protein -
  EXW62_RS19790 (EXW62_20245) - 3905313..3905555 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW62_RS19795 (EXW62_20250) - 3905663..3907057 (+) 1395 WP_215554193.1 L-cystine transporter -
  EXW62_RS19800 (EXW62_20255) - 3907138..3907530 (-) 393 WP_071747138.1 hypothetical protein -
  EXW62_RS19805 (EXW62_20260) - 3907617..3907832 (-) 216 WP_002160480.1 DUF3912 family protein -
  EXW62_RS19810 (EXW62_20265) - 3908085..3908396 (+) 312 WP_002111840.1 hypothetical protein -
  EXW62_RS19815 (EXW62_20270) - 3908429..3908917 (-) 489 WP_215554194.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39141.40 Da        Isoelectric Point: 8.4517

>NTDB_id=346090 EXW62_RS19780 WP_016106153.1 3903236..3904279(-) (comGA) [Bacillus mycoides strain BPN57/2]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGETRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346090 EXW62_RS19780 WP_016106153.1 3903236..3904279(-) (comGA) [Bacillus mycoides strain BPN57/2]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAACGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTAGTTATTCGTCT
CCATTTACAAGCATCTGTTCAGCCATTGTCTCATCTTTCGTTATTTCCAAGTACAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGGAAAACGACGACAATGTACGCGTTATTAGAAGTA
GCTAGAAAAGGAGAAACACGTCGTATTGTTACTCTGGAAGATCCAGTTGAGAGAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATGAAACGGGATTAAAGGCTATTTTGCGTCACGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCGAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTGATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGACTCGTTGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGAC
AAGTGAGGCAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTGCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G4EMB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Haemophilus influenzae 86-028NP

38.82

92.795

0.36
  pilB Glaesserella parasuis strain SC1401

43.706

82.421

0.36

Multiple sequence alignment