Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   PaeAG1_RS30845 Genome accession   NZ_CP045739
Coordinates   6526276..6526872 (-) Length   198 a.a.
NCBI ID   WP_015647905.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6521276..6531872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PaeAG1_RS30825 (PaeAG1_06103) aroK 6522407..6522925 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PaeAG1_RS30830 (PaeAG1_06104) pilQ 6522937..6525081 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  PaeAG1_RS30835 (PaeAG1_06105) comL/pilP 6525135..6525659 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PaeAG1_RS30840 (PaeAG1_06106) pilO 6525656..6526279 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PaeAG1_RS30845 (PaeAG1_06107) pilN 6526276..6526872 (-) 597 WP_015647905.1 type 4a pilus biogenesis protein PilN Machinery gene
  PaeAG1_RS30850 (PaeAG1_06108) pilM 6526872..6527936 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PaeAG1_RS30855 (PaeAG1_06109) - 6528121..6530589 (+) 2469 WP_025297613.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22199.52 Da        Isoelectric Point: 10.0895

>NTDB_id=345677 PaeAG1_RS30845 WP_015647905.1 6526276..6526872(-) (pilN) [Pseudomonas aeruginosa strain AG1]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAVENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=345677 PaeAG1_RS30845 WP_015647905.1 6526276..6526872(-) (pilN) [Pseudomonas aeruginosa strain AG1]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCGTCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ATAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.143

88.384

0.364

Multiple sequence alignment