Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW31_RS20600 Genome accession   NZ_CP035974
Coordinates   3938528..3939571 (-) Length   347 a.a.
NCBI ID   WP_016106153.1    Uniprot ID   A0A1G4EMB3
Organism   Bacillus mycoides strain BPN102     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3933528..3944571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW31_RS20555 (EXW31_20910) - 3933926..3934126 (-) 201 WP_002015009.1 YqzE family protein -
  EXW31_RS20560 (EXW31_20915) - 3934165..3934662 (-) 498 WP_002160495.1 shikimate kinase -
  EXW31_RS20565 (EXW31_20920) - 3934815..3935465 (-) 651 WP_016106149.1 2OG-Fe(II) oxygenase -
  EXW31_RS20570 (EXW31_20925) comGG 3935639..3936010 (-) 372 WP_016106150.1 competence type IV pilus minor pilin ComGG -
  EXW31_RS20575 (EXW31_20930) comGF 3936007..3936468 (-) 462 WP_078181634.1 competence type IV pilus minor pilin ComGF -
  EXW31_RS20580 (EXW31_20935) - 3936452..3936748 (-) 297 WP_002160491.1 hypothetical protein -
  EXW31_RS20585 (EXW31_20940) comGD 3936741..3937196 (-) 456 WP_002160490.1 competence type IV pilus minor pilin ComGD -
  EXW31_RS20590 (EXW31_20945) comGC 3937193..3937492 (-) 300 WP_002160489.1 competence type IV pilus major pilin ComGC -
  EXW31_RS20595 (EXW31_20950) comGB 3937504..3938541 (-) 1038 WP_078172815.1 competence type IV pilus assembly protein ComGB -
  EXW31_RS20600 (EXW31_20955) comGA 3938528..3939571 (-) 1044 WP_016106153.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW31_RS20605 (EXW31_20960) - 3939777..3940472 (+) 696 WP_078172814.1 helix-turn-helix transcriptional regulator -
  EXW31_RS20610 (EXW31_20965) - 3940605..3940847 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW31_RS20615 (EXW31_20970) - 3940955..3942349 (+) 1395 WP_215572041.1 L-cystine transporter -
  EXW31_RS20620 (EXW31_20975) - 3942593..3942988 (-) 396 WP_033707183.1 hypothetical protein -
  EXW31_RS20625 (EXW31_20980) - 3943076..3943291 (-) 216 WP_002067106.1 DUF3912 family protein -
  EXW31_RS20630 (EXW31_20985) - 3943544..3943855 (+) 312 WP_002160479.1 hypothetical protein -
  EXW31_RS20635 (EXW31_20990) - 3943889..3944377 (-) 489 WP_002160478.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39141.40 Da        Isoelectric Point: 8.4517

>NTDB_id=345425 EXW31_RS20600 WP_016106153.1 3938528..3939571(-) (comGA) [Bacillus mycoides strain BPN102]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGETRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345425 EXW31_RS20600 WP_016106153.1 3938528..3939571(-) (comGA) [Bacillus mycoides strain BPN102]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAAAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAAAGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTAGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCGTTGTCTCATCTTTCGTTATTTCCAAGTACAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACGACAATGTATGCGTTATTAGAAGTA
GCAAGAAAAGGAGAAACACGTCGCATCGTTACGCTGGAAGATCCAGTTGAGAGAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATGAAACGGGATTAAAGGCTATTTTGCGTCACGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTGATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGACTCGTTGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGGCAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTGCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G4EMB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Haemophilus influenzae 86-028NP

38.82

92.795

0.36
  pilB Glaesserella parasuis strain SC1401

43.706

82.421

0.36

Multiple sequence alignment