Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW30_RS20330 Genome accession   NZ_CP035962
Coordinates   3974415..3975458 (-) Length   347 a.a.
NCBI ID   WP_016106153.1    Uniprot ID   A0A1G4EMB3
Organism   Bacillus mycoides strain BPN07/3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3969415..3980458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW30_RS20285 (EXW30_20665) - 3969813..3970013 (-) 201 WP_002015009.1 YqzE family protein -
  EXW30_RS20290 (EXW30_20670) - 3970052..3970549 (-) 498 WP_215569190.1 shikimate kinase -
  EXW30_RS20295 (EXW30_20675) - 3970701..3971351 (-) 651 WP_016106149.1 2OG-Fe(II) oxygenase -
  EXW30_RS20300 (EXW30_20680) comGG 3971525..3971896 (-) 372 WP_016106150.1 competence type IV pilus minor pilin ComGG -
  EXW30_RS20305 (EXW30_20685) comGF 3971893..3972354 (-) 462 WP_002160492.1 competence type IV pilus minor pilin ComGF -
  EXW30_RS20310 (EXW30_20690) - 3972338..3972634 (-) 297 WP_002160491.1 hypothetical protein -
  EXW30_RS20315 (EXW30_20695) comGD 3972627..3973082 (-) 456 WP_002160490.1 competence type IV pilus minor pilin ComGD -
  EXW30_RS20320 (EXW30_20700) comGC 3973079..3973378 (-) 300 WP_215569192.1 competence type IV pilus major pilin ComGC -
  EXW30_RS20325 (EXW30_20705) comGB 3973391..3974428 (-) 1038 WP_078172815.1 competence type IV pilus assembly protein ComGB -
  EXW30_RS20330 (EXW30_20710) comGA 3974415..3975458 (-) 1044 WP_016106153.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW30_RS20335 (EXW30_20715) - 3975664..3976359 (+) 696 WP_078172814.1 helix-turn-helix transcriptional regulator -
  EXW30_RS20340 (EXW30_20720) - 3976492..3976734 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW30_RS20345 (EXW30_20725) - 3976842..3978236 (+) 1395 WP_215569194.1 L-cystine transporter -
  EXW30_RS20350 (EXW30_20730) - 3978315..3978707 (-) 393 WP_016106155.1 hypothetical protein -
  EXW30_RS20355 (EXW30_20735) - 3978794..3979009 (-) 216 WP_002160480.1 DUF3912 family protein -
  EXW30_RS20360 (EXW30_20740) - 3979262..3979573 (+) 312 WP_002160479.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39141.40 Da        Isoelectric Point: 8.4517

>NTDB_id=345326 EXW30_RS20330 WP_016106153.1 3974415..3975458(-) (comGA) [Bacillus mycoides strain BPN07/3]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGETRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345326 EXW30_RS20330 WP_016106153.1 3974415..3975458(-) (comGA) [Bacillus mycoides strain BPN07/3]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAAAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAAAGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTAGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCGTTGTCTCATCTTTCGTTATTTCCAAGTACAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACGACAATGTATGCGTTATTAGAAGTA
GCAAGAAAAGGAGAAACACGTCGCATCGTTACGCTGGAAGATCCAGTTGAGAGAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATGAAACGGGATTAAAGGCTATTTTGCGTCACGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTGATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGACTCGTTGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGGCAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTGCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G4EMB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Haemophilus influenzae 86-028NP

38.82

92.795

0.36
  pilB Glaesserella parasuis strain SC1401

43.706

82.421

0.36

Multiple sequence alignment