Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW58_RS19625 Genome accession   NZ_CP035953
Coordinates   3819712..3820755 (-) Length   347 a.a.
NCBI ID   WP_088079369.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN03/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3814712..3825755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW58_RS19580 (EXW58_19855) - 3815111..3815311 (-) 201 WP_002015009.1 YqzE family protein -
  EXW58_RS19585 (EXW58_19860) - 3815350..3815847 (-) 498 WP_002160495.1 shikimate kinase -
  EXW58_RS19590 (EXW58_19865) - 3815999..3816649 (-) 651 WP_078172817.1 2OG-Fe(II) oxygenase -
  EXW58_RS19595 (EXW58_19870) comGG 3816823..3817194 (-) 372 WP_215579658.1 competence type IV pilus minor pilin ComGG -
  EXW58_RS19600 (EXW58_19875) comGF 3817191..3817652 (-) 462 WP_088079367.1 competence type IV pilus minor pilin ComGF -
  EXW58_RS19605 (EXW58_19880) - 3817636..3817932 (-) 297 WP_002160491.1 hypothetical protein -
  EXW58_RS19610 (EXW58_19885) comGD 3817925..3818380 (-) 456 WP_002160490.1 competence type IV pilus minor pilin ComGD -
  EXW58_RS19615 (EXW58_19890) comGC 3818377..3818676 (-) 300 WP_215579660.1 competence type IV pilus major pilin ComGC -
  EXW58_RS19620 (EXW58_19895) comGB 3818688..3819725 (-) 1038 WP_078172815.1 competence type IV pilus assembly protein ComGB -
  EXW58_RS19625 (EXW58_19900) comGA 3819712..3820755 (-) 1044 WP_088079369.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW58_RS19630 (EXW58_19905) - 3820961..3821656 (+) 696 WP_215579662.1 helix-turn-helix domain-containing protein -
  EXW58_RS19635 (EXW58_19910) - 3821791..3822033 (+) 243 WP_002088627.1 DUF2626 domain-containing protein -
  EXW58_RS19640 (EXW58_19915) - 3822141..3823535 (+) 1395 WP_002160483.1 L-cystine transporter -
  EXW58_RS19645 (EXW58_19920) - 3823614..3824006 (-) 393 WP_016106155.1 hypothetical protein -
  EXW58_RS19650 (EXW58_19925) - 3824093..3824308 (-) 216 WP_002160480.1 DUF3912 family protein -
  EXW58_RS19655 (EXW58_19930) - 3824561..3824872 (+) 312 WP_002160479.1 hypothetical protein -
  EXW58_RS19660 (EXW58_19935) - 3824907..3825395 (-) 489 WP_215579664.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39140.46 Da        Isoelectric Point: 8.8464

>NTDB_id=345278 EXW58_RS19625 WP_088079369.1 3819712..3820755(-) (comGA) [Bacillus mycoides strain BPN03/1]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAKKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGETRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=345278 EXW58_RS19625 WP_088079369.1 3819712..3820755(-) (comGA) [Bacillus mycoides strain BPN03/1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAAAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAAAGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTAGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCGTTGTCTCATCTTTCGTTATTTCCAAGTACAGCGAAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACGACAATGTATGCGTTATTAGAAGTA
GCAAGAAAAGGAGAAACACGTCGCATCGTTACGCTGGAAGATCCAGTTGAGAGAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATGAAACGGGATTAAAGGCTATTTTGCGTCACGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTGATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGACTCGTTGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGGCAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTGCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Haemophilus influenzae 86-028NP

38.82

92.795

0.36
  pilB Glaesserella parasuis strain SC1401

43.706

82.421

0.36

Multiple sequence alignment