Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EXW58_RS05570 Genome accession   NZ_CP035953
Coordinates   1093930..1097655 (+) Length   1241 a.a.
NCBI ID   WP_215580884.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN03/1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1088930..1102655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW58_RS05555 (EXW58_05635) - 1089087..1089680 (+) 594 WP_002158925.1 TVP38/TMEM64 family protein -
  EXW58_RS05560 (EXW58_05640) lepB 1089737..1090300 (+) 564 WP_016104147.1 signal peptidase I -
  EXW58_RS05565 (EXW58_05645) addB 1090418..1093933 (+) 3516 WP_215580882.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW58_RS05570 (EXW58_05650) addA 1093930..1097655 (+) 3726 WP_215580884.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW58_RS05575 (EXW58_05655) - 1097679..1097957 (+) 279 WP_215580886.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW58_RS05580 (EXW58_05660) - 1098175..1098882 (+) 708 WP_215580887.1 DNA alkylation repair protein -
  EXW58_RS05585 (EXW58_05665) gerPF 1098934..1099149 (-) 216 WP_002170786.1 spore germination protein GerPF -
  EXW58_RS05590 (EXW58_05670) - 1099192..1099578 (-) 387 WP_044437740.1 spore germination protein GerPE -
  EXW58_RS05595 (EXW58_05675) - 1099593..1099787 (-) 195 WP_113734011.1 spore gernimation protein GerPD -
  EXW58_RS05600 (EXW58_05680) - 1099794..1100408 (-) 615 WP_002158914.1 spore germination protein GerPC -
  EXW58_RS05605 (EXW58_05685) gerPB 1100473..1100679 (-) 207 WP_002149891.1 spore germination protein GerPB -
  EXW58_RS05610 (EXW58_05690) gerPA 1100694..1100915 (-) 222 WP_002149890.1 spore germination protein GerPA -
  EXW58_RS05615 (EXW58_05695) - 1101012..1101191 (-) 180 WP_002087032.1 aspartyl-phosphate phosphatase Spo0E family protein -
  EXW58_RS05620 (EXW58_05700) - 1101435..1102337 (+) 903 WP_016104153.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142734.87 Da        Isoelectric Point: 4.8340

>NTDB_id=345258 EXW58_RS05570 WP_215580884.1 1093930..1097655(+) (addA) [Bacillus mycoides strain BPN03/1]
MIENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIGNQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEEHI
RKATELAMLPDGPAPRVETLQADVALLGMLSSAARGSWTNVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYGYSASWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWEYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGRRVLAAKSVEREVPFTMMLSAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPTL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIDE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=345258 EXW58_RS05570 WP_215580884.1 1093930..1097655(+) (addA) [Bacillus mycoides strain BPN03/1]
ATGATAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCGGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACCATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGGGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGACGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAGTTTCAACTTGAAACAGCGGAAGAGCATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACGTTACAAGCGGATGTAGCTTTACT
TGGAATGTTATCCTCAGCAGCTCGCGGGTCGTGGACAAACGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGCTAA
AGCGTATTAAGAAAAGTGATTACAACGAAGATGTTGTAAAGCAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAACTATTTAGCCGCAGACCTGAAAGTTTCTTGCGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGTGAGCAAAGTGAAGATGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATTGGACGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTATGCA
TTCAGCAAACGGAAGAAGAAGTGCAAGACGGTGAAGAAGGTACAGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTCGATTCAGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCAGTGCAATA
CCGTGATTTCGTTATTTTACTTCGCTCCATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCGG
TATATGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGTTCATTCTTAAAAGGAGCGCCGCTTGAAGAAGAACAAGAAT
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCGCGTCAACAATCTCTTTCCGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
ACGCGTATTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGATTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGACATGGGAACGGCGAGAGCTCTTGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCATGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGGAATATTCCAGGCGAAATTTATGGGTATAGTGCGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAGTTATTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTATATGACAGATTAATGTGGGAATACGGGTATGAGG
ACGCAACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAGAAACTCCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAGCAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAGAAGGGTATTAGCAGCAAAAAGTGTTGAACGTGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGTAAGAGCGACGAAACAATACTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGATG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTCCCAGGCGGATTTGATCAAGCGAAACCAACTTTA
GAAGACCGATACAAAGTGCAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAATATTGACGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.408

100

0.537

Multiple sequence alignment