Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW58_RS05565 Genome accession   NZ_CP035953
Coordinates   1090418..1093933 (+) Length   1171 a.a.
NCBI ID   WP_215580882.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN03/1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1085418..1098933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW58_RS05530 (EXW58_05610) - 1085420..1085989 (+) 570 WP_215580878.1 glycosyltransferase family A protein -
  EXW58_RS05535 (EXW58_05615) - 1086139..1086912 (+) 774 WP_215580880.1 FkbM family methyltransferase -
  EXW58_RS05540 (EXW58_05620) - 1086936..1087094 (-) 159 Protein_997 helix-turn-helix domain-containing protein -
  EXW58_RS05545 (EXW58_05625) - 1087305..1087886 (+) 582 WP_204209402.1 competence protein ComK -
  EXW58_RS05550 (EXW58_05630) - 1088131..1088729 (+) 599 Protein_999 DUF4306 domain-containing protein -
  EXW58_RS05555 (EXW58_05635) - 1089087..1089680 (+) 594 WP_002158925.1 TVP38/TMEM64 family protein -
  EXW58_RS05560 (EXW58_05640) lepB 1089737..1090300 (+) 564 WP_016104147.1 signal peptidase I -
  EXW58_RS05565 (EXW58_05645) addB 1090418..1093933 (+) 3516 WP_215580882.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW58_RS05570 (EXW58_05650) addA 1093930..1097655 (+) 3726 WP_215580884.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW58_RS05575 (EXW58_05655) - 1097679..1097957 (+) 279 WP_215580886.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW58_RS05580 (EXW58_05660) - 1098175..1098882 (+) 708 WP_215580887.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134270.34 Da        Isoelectric Point: 5.2129

>NTDB_id=345257 EXW58_RS05565 WP_215580882.1 1090418..1093933(+) (addB) [Bacillus mycoides strain BPN03/1]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGVEIYIDGFHSFSPQELEIVRGLMRLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYERIKQVAREEKIEVEKTIPLMGQPRFHSQALAHLEMHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVAAEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMTTGLEALQFANIPPSLDQVLVANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEERNVLNAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=345257 EXW58_RS05565 WP_215580882.1 1090418..1093933(+) (addB) [Bacillus mycoides strain BPN03/1]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGGA
GCGAAGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTGCTTCGTAAAATTGTAGAATCTCGTAAAGATGGACT
ATCTGTGTTTCAAAAAGCAGCGGAGCAAAATGGCTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCGTCTAACGTATATGAAATGTGGCAACAATTAGATACGCATAGCAGTAGTGCAGAACAAAAGCTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGTGGAAATTTATATAGATGGATTCCATTCAT
TTTCTCCTCAAGAGCTAGAAATTGTTAGAGGGCTAATGAGATTAGGGACTAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTGGATTTATTTTATGAAACGACGTTAACGTATGAAAGAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAGTTGAAAAAACAATTCCACTTATGGGACAGCCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCGAGTGTGACAATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGAAGACTTGTGGCGGCTGAAGACTATCGTTACCGAGATATCGCAGT
ACTTCTTCGTAACGGAGAAAGTTATTACGATGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGTATTCGTTCGGCTTTAGAGATTATTAGCGGAAATTGGCGTTAT
GATGCGGTGTTCCGCTGTGTGAAGACAGAACTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAAAGCGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAGCCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTGTCTATGTTTACTGATGTAATGACGACAGGTCTTGAGGCGCTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAATATTGATCATTCTAGATTATCAGATGTTAAAGCGACGTTCATTATTGGCGTAAATGAAGGAG
TGATTCCAGCAGCGCCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCAACGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCA
GAAGTAACGTTGTCTTATGTCATGCAGCAACTGCAAACGTGGAAGCGTTACGGATTTGAAGGCAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTGTTATCGAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTTGCTCAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGCTTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGTGGTACTGGATCGCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGCGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGACTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGCAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.148

100

0.493

Multiple sequence alignment