Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EPB58_RS17680 Genome accession   NZ_CP035693
Coordinates   1628163..1628738 (+) Length   191 a.a.
NCBI ID   WP_005451499.1    Uniprot ID   A0A9X3MLM3
Organism   Vibrio harveyi strain 2011V-1164     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1623163..1633738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB58_RS17665 oxyR 1623439..1624347 (+) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  EPB58_RS17670 - 1624414..1626984 (-) 2571 WP_050935720.1 PBP1A family penicillin-binding protein -
  EPB58_RS17675 pilM 1627163..1628179 (+) 1017 WP_154203380.1 type IV pilus assembly protein PilM Machinery gene
  EPB58_RS17680 pilN 1628163..1628738 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  EPB58_RS17685 pilO 1628731..1629324 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB58_RS17690 pilP 1629314..1629829 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  EPB58_RS17695 pilQ 1629864..1631621 (+) 1758 WP_195724146.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB58_RS17700 aroK 1631811..1632329 (+) 519 WP_005451507.1 shikimate kinase AroK -
  EPB58_RS17705 aroB 1632361..1633461 (+) 1101 WP_038898114.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22244.63 Da        Isoelectric Point: 9.1586

>NTDB_id=343782 EPB58_RS17680 WP_005451499.1 1628163..1628738(+) (pilN) [Vibrio harveyi strain 2011V-1164]
MLYHINLLAWRENQREEHRRRFLSLIVLGVIVALGVQWGIGKFYQYQQDQQQQRLDYLAHYISQLDKRIEAMKIAEQEHS
KILERLKIVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTPRLATMLDNMERSAKLLDVDMHSIV
HGKARFGKEFQTFKVSFMFKDASVKGGNQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=343782 EPB58_RS17680 WP_005451499.1 1628163..1628738(+) (pilN) [Vibrio harveyi strain 2011V-1164]
ATGCTGTATCATATTAACCTCCTTGCGTGGCGTGAGAATCAACGTGAAGAACACCGCCGCCGCTTTCTCAGTTTGATCGT
CTTGGGGGTGATTGTTGCGTTGGGCGTGCAATGGGGCATTGGTAAGTTCTATCAATATCAGCAGGATCAACAGCAGCAGC
GCCTTGATTATTTGGCGCACTATATCTCCCAGCTTGATAAACGAATCGAAGCGATGAAGATTGCTGAACAAGAGCACAGC
AAGATCTTGGAGAGGTTAAAGATCGTTGAAGGTTTGCAAAATGGGCGTAATAAAACCACGGAGTTCATGAACCTGATGCC
GTCGGTGATCCCTGAAGGAGTCTACGTCGATAAGATCAAAATGAATGATTATGAAATTGAAATCTCGGGTATCTCTGATA
GTACACCTCGCTTGGCCACCATGCTCGACAACATGGAGCGTTCAGCCAAGCTGCTAGATGTCGACATGCATTCTATCGTT
CATGGTAAAGCGCGTTTTGGGAAAGAGTTTCAGACTTTCAAAGTTTCCTTCATGTTCAAAGACGCCTCGGTGAAGGGAGG
TAACCAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

94.241

100

0.942
  pilN Vibrio cholerae strain A1552

60.452

92.67

0.56

Multiple sequence alignment