Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EPB58_RS17675 Genome accession   NZ_CP035693
Coordinates   1627163..1628179 (+) Length   338 a.a.
NCBI ID   WP_154203380.1    Uniprot ID   -
Organism   Vibrio harveyi strain 2011V-1164     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1622163..1633179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB58_RS17660 - 1622536..1623264 (-) 729 WP_005451494.1 redoxin family protein -
  EPB58_RS17665 oxyR 1623439..1624347 (+) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  EPB58_RS17670 - 1624414..1626984 (-) 2571 WP_050935720.1 PBP1A family penicillin-binding protein -
  EPB58_RS17675 pilM 1627163..1628179 (+) 1017 WP_154203380.1 type IV pilus assembly protein PilM Machinery gene
  EPB58_RS17680 pilN 1628163..1628738 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  EPB58_RS17685 pilO 1628731..1629324 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB58_RS17690 pilP 1629314..1629829 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  EPB58_RS17695 pilQ 1629864..1631621 (+) 1758 WP_195724146.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB58_RS17700 aroK 1631811..1632329 (+) 519 WP_005451507.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38049.44 Da        Isoelectric Point: 6.3149

>NTDB_id=343781 EPB58_RS17675 WP_154203380.1 1627163..1628179(+) (pilM) [Vibrio harveyi strain 2011V-1164]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDGYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSY

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=343781 EPB58_RS17675 WP_154203380.1 1627163..1628179(+) (pilM) [Vibrio harveyi strain 2011V-1164]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACG
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTTGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAGGTTGCTACCGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCATATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.237

100

0.902
  pilM Vibrio cholerae strain A1552

48.83

100

0.494

Multiple sequence alignment