Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   F8M43_RS09035 Genome accession   NZ_CP044498
Coordinates   1728845..1731421 (+) Length   858 a.a.
NCBI ID   WP_029726481.1    Uniprot ID   -
Organism   Bacillus subtilis strain ms-2     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1723845..1736421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS09015 kbl 1724617..1725795 (+) 1179 WP_014664025.1 glycine C-acetyltransferase -
  F8M43_RS09020 miaB 1725943..1727472 (+) 1530 WP_014479820.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  F8M43_RS09025 ymcA 1727474..1727905 (+) 432 WP_003231834.1 regulatory iron-sulfur-containing complex subunit RicA Regulator
  F8M43_RS09030 cotE 1728167..1728712 (+) 546 WP_003231833.1 outer spore coat protein CotE -
  F8M43_RS09035 hexA 1728845..1731421 (+) 2577 WP_029726481.1 DNA mismatch repair protein MutS Machinery gene
  F8M43_RS09040 hexB 1731437..1733320 (+) 1884 WP_029726480.1 DNA mismatch repair endonuclease MutL Machinery gene
  F8M43_RS09045 ymzD 1733719..1734174 (-) 456 WP_015252068.1 regulatory YrvL family protein -
  F8M43_RS09050 - 1734330..1734887 (-) 558 WP_029726479.1 hypothetical protein -
  F8M43_RS09055 pksA 1735007..1735624 (+) 618 WP_029317807.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 858 a.a.        Molecular weight: 97589.66 Da        Isoelectric Point: 5.1585

>NTDB_id=343211 F8M43_RS09035 WP_029726481.1 1728845..1731421(+) (hexA) [Bacillus subtilis strain ms-2]
MAGYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGAAEKIPMCGVPYHSASAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSACSNGYGLALSDLTTGENLAVLIERLEDV
ISEIYSVGAREIVVSGSLDADTVAQLRERCGATISIEDGETDEHVTIIEHLNNEDITKTFLRLYTYLKRTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKNKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRVNQIEERQEMVETLMSHFF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPGIKQLVASLAHDKAKERAERIDPCGDVLELLEEALYE
NPPLSVKEGNLIKDGYNQKLDEYRDASRNGKDWIARLEQQEREYTGIRSLKVGFNKVFGYYIEVTKANLHLLEEGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFTELREKVKQYIPRLQLLAKQMSELDALQCFATISENRHYTKPEF
SKDEVEVIEGRHPVVEKVMDSQEYVPNNCMMGDNRQMLLITGPNMSGKSTYMRQIALISIMAQIGCFVPAKKAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKNSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPGDLIARAQDILKELEHSGNKPEVPVQKPQV
KEEPAQLSFFDEAEKPAETPKLSKKEKQVIDAFKSLNILDMTPLEAMNEMYKLQKKLH

Nucleotide


Download         Length: 2577 bp        

>NTDB_id=343211 F8M43_RS09035 WP_029726481.1 1728845..1731421(+) (hexA) [Bacillus subtilis strain ms-2]
ATGGCCGGTTATACGCCTATGATACAGCAATATTTAAAAATAAAGGCAGAGCACCAGGATGCCTTTTTATTTTTTCGCCT
GGGTGATTTTTACGAAATGTTTTTTGAGGACGCCAAAAAAGCGTCACAAGAGCTGGAAATTACGTTAACGAGCAGAGATG
GCGGTGCGGCTGAAAAAATACCGATGTGCGGTGTGCCGTATCATTCTGCTTCCGCGTATATCGAACAGCTTATTAAAAAA
GGATACAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAAGCCGCAAAGGGCGTTGTGAAAAGAGAAGTGGTTCAGCT
GATTACGCCCGGAACTGTAATGGACGGCAAAGGCATCCATGAATCGGAAAATAACTTTATCGCATCTGTTTCAGCCTGCT
CGAACGGATACGGACTTGCGCTGTCTGATTTAACAACGGGAGAAAATTTGGCTGTTTTGATTGAACGGCTTGAAGACGTC
ATATCAGAAATTTATTCAGTCGGCGCACGGGAAATCGTGGTTTCAGGAAGCTTGGATGCCGATACAGTGGCACAGCTGAG
AGAGCGGTGCGGTGCAACAATTTCAATTGAAGACGGAGAAACAGACGAACACGTAACGATCATTGAGCACTTAAATAATG
AAGATATAACAAAAACATTTCTGCGCTTATATACGTATTTGAAAAGAACCCAAAAGCGCAGCCTTGATCATCTTCAGCCC
GTGCAGGTGTATGAGCTTGAGGAAGCGATGAAAATTGACTTGTACTCAAAGCGTAATCTGGAGCTGACCGAAACGATTCG
TTCGAAAAATAAAAAAGGCTCCCTTTTGTGGCTGCTGGATGAAACAAAAACGGCAATGGGAGGCCGGCTGCTTAAACAGT
GGATTGACCGGCCGCTTATCAGAGTCAATCAAATCGAAGAGCGCCAAGAAATGGTGGAAACATTAATGTCCCATTTCTTT
GAACGGGAAGATCTGCGTGAACGTTTAAAAGAAGTATATGACTTAGAACGCCTTGCCGGCCGCGTTGCATTCGGAAATGT
CAATGCCCGGGATTTGATTCAGCTGAAGGAATCGTTAAAGCAAGTTCCTGGCATCAAACAGCTGGTTGCTTCACTGGCTC
ATGACAAGGCCAAGGAACGTGCAGAGCGCATTGACCCTTGCGGGGATGTACTTGAACTGCTGGAAGAAGCGCTGTACGAA
AACCCTCCTTTATCGGTAAAAGAAGGGAATCTGATTAAAGATGGTTATAATCAAAAGCTTGATGAATATCGTGATGCAAG
CAGAAACGGAAAAGACTGGATTGCCCGCCTGGAACAGCAGGAGCGGGAATACACAGGCATTCGTTCTTTAAAGGTTGGCT
TTAATAAAGTTTTCGGTTATTACATTGAAGTGACAAAAGCAAATTTGCATTTGCTTGAGGAAGGGCGTTATGAGCGGAAA
CAGACGTTAACCAATGCAGAGCGCTACATTACACCTGAATTAAAAGAAAAAGAAGCGCTCATTTTAGAGGCGGAAAATAA
CATCTGTGAGCTGGAGTACGAGCTGTTTACCGAGCTGCGCGAGAAAGTGAAGCAGTATATTCCGCGGCTACAGTTGCTTG
CAAAACAGATGAGCGAGCTGGACGCACTGCAATGCTTTGCGACAATCAGTGAAAATCGCCACTACACGAAACCGGAGTTC
TCTAAAGATGAAGTCGAAGTGATTGAAGGCAGACACCCGGTTGTTGAAAAAGTCATGGACAGCCAGGAATATGTCCCGAA
CAACTGTATGATGGGCGATAACAGACAAATGCTTCTCATTACAGGTCCAAACATGTCAGGGAAAAGCACGTATATGAGAC
AAATCGCGCTCATTTCCATTATGGCGCAAATCGGCTGCTTTGTACCCGCGAAAAAAGCGGTGCTTCCGATTTTTGATCAA
ATTTTCACACGAATCGGCGCTGCAGATGATTTGATTTCCGGACAAAGTACATTTATGGTAGAAATGCTTGAAGCGAAAAA
TGCGATTGTCAACGCGACGAAAAACAGCCTCATTCTGTTTGATGAAATCGGGCGGGGAACGTCCACTTATGACGGCATGG
CGCTGGCACAGGCGATTATTGAATATGTTCACGATCATATCGGCGCCAAGACGCTGTTCAGCACACACTATCACGAGCTG
ACCGTTCTTGAGGACAAGCTGCCCCAGCTGAAAAACGTTCATGTTCGCGCTGAAGAATATAACGGAACGGTTGTCTTTCT
TCATCAAATCAAAGAAGGTGCCGCTGATAAAAGCTATGGTATCCATGTAGCTCAGCTTGCCGAACTGCCGGGAGATCTGA
TTGCCCGCGCCCAAGATATTTTAAAAGAGCTTGAACATTCAGGAAACAAACCGGAAGTGCCGGTGCAGAAACCTCAGGTG
AAAGAAGAGCCGGCACAGCTGTCCTTTTTTGACGAAGCGGAAAAGCCGGCTGAAACACCAAAGCTTTCAAAAAAAGAAAA
GCAAGTGATCGATGCTTTCAAATCACTTAATATACTTGATATGACACCGCTTGAAGCGATGAATGAAATGTACAAGCTGC
AAAAGAAATTACATTAA

Domains

Predicted by InterProScan.

MutS domain III (MutS_III)

(258-547)

MutS domain V (MutS_V)

(598-785)

MutS family domain IV (MutS_IV)

(415-506)

MutS domain II (MutS_II)

(125-239)

MutS domain I (MutS_I)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

49.474

99.65

0.493
  mutS Pseudomonas stutzeri strain ATCC 17587

38.32

100

0.388