Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   EU243_RS12130 Genome accession   NZ_CP035533
Coordinates   2358589..2359389 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain DTU001     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2353589..2364389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EU243_RS12110 (EU243_12300) - 2353712..2354557 (+) 846 WP_015388017.1 TlyA family RNA methyltransferase -
  EU243_RS12115 (EU243_12305) ahrC 2354654..2355103 (+) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  EU243_RS12120 (EU243_12310) recN 2355135..2356864 (+) 1730 Protein_2345 DNA repair protein RecN -
  EU243_RS12125 (EU243_12315) spoIVB 2357028..2358308 (+) 1281 WP_007408360.1 SpoIVB peptidase -
  EU243_RS12130 (EU243_12320) spo0A 2358589..2359389 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  EU243_RS12135 (EU243_12325) alr 2359535..2360716 (-) 1182 WP_015388018.1 alanine racemase -
  EU243_RS12140 (EU243_12330) - 2360890..2361102 (-) 213 WP_014305392.1 spore germination protein -
  EU243_RS12145 (EU243_12335) - 2361228..2361440 (-) 213 WP_003153183.1 spore germination protein -
  EU243_RS12150 (EU243_12340) - 2361566..2362684 (-) 1119 WP_043867644.1 NADH-dependent flavin oxidoreductase -
  EU243_RS12155 - 2362778..2362915 (-) 138 WP_014470646.1 hypothetical protein -
  EU243_RS12160 (EU243_12345) - 2363206..2363838 (-) 633 WP_014418348.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=343010 EU243_RS12130 WP_003153177.1 2358589..2359389(+) (spo0A) [Bacillus velezensis strain DTU001]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=343010 EU243_RS12130 WP_003153177.1 2358589..2359389(+) (spo0A) [Bacillus velezensis strain DTU001]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCCC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment