Detailed information NTG0342939

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EI683_RS01025
• Genome accession: NZ_CP035531
• Coordinates: 216825..217625 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain NFYY0065
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 211825..222625

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EI683_RS01005 (EI683_01010)	dksA	212405..212860 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
EI683_RS01010 (EI683_01015)	sfsA	213023..213739 (-)	717	WP_023342992.1	DNA/RNA nuclease SfsA	-
EI683_RS01015 (EI683_01020)	thpR	213739..214275 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
EI683_RS01020 (EI683_01025)	hrpB	214348..216777 (+)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
EI683_RS01025 (EI683_01030)	pilD	216825..217625 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
EI683_RS01030 (EI683_01035)	-	217622..218368 (-)	747	WP_023316460.1	type II secretion system protein N	-
EI683_RS01035 (EI683_01040)	-	218358..218843 (-)	486	WP_004151943.1	type II secretion system protein M	-
EI683_RS01040 (EI683_01045)	gspL	218836..220029 (-)	1194	WP_032428212.1	type II secretion system protein GspL	-
EI683_RS01045 (EI683_01050)	gspK	220016..221002 (-)	987	WP_023342990.1	type II secretion system minor pseudopilin GspK	-
EI683_RS01050 (EI683_01055)	gspJ	220999..221595 (-)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
EI683_RS01055 (EI683_01060)	gspI	221592..221957 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
EI683_RS01060 (EI683_01065)	gspH	221954..222469 (-)	516	WP_021313445.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=342939 EI683_RS01025 WP_004145898.1 216825..217625(-) (pilD) [Klebsiella pneumoniae strain NFYY0065]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=342939 EI683_RS01025 WP_004145898.1 216825..217625(-) (pilD) [Klebsiella pneumoniae strain NFYY0065]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425