Detailed information NTG0342261

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BACIH_RS07840
• Genome accession: NZ_CP044360
• Coordinates: 1531955..1532944 (+)
• Length: 329 a.a.
• NCBI ID: WP_012117545.1
• Uniprot ID: A7Z4L5
• Organism: Bacillus amyloliquefaciens strain V167
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1526955..1537944

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BACIH_RS07825 (BACIH_1592)	acpP	1527151..1527384 (+)	234	WP_003154310.1	acyl carrier protein	-
BACIH_RS07830 (BACIH_1593)	rncS	1527523..1528272 (+)	750	WP_012117543.1	ribonuclease III	-
BACIH_RS07835 (BACIH_1595)	smc	1528374..1531935 (+)	3562	Protein_1505	chromosome segregation protein SMC	-
BACIH_RS07840 (BACIH_1596)	pilA	1531955..1532944 (+)	990	WP_012117545.1	signal recognition particle-docking protein FtsY	Machinery gene
BACIH_RS07845 (BACIH_1597)	-	1533028..1533798 (+)	771	WP_012117546.1	phosphotransferase family protein	-
BACIH_RS07850 (BACIH_1598)	-	1533920..1534252 (+)	333	WP_003154299.1	putative DNA-binding protein	-
BACIH_RS07855 (BACIH_1599)	ffh	1534266..1535607 (+)	1342	Protein_1509	signal recognition particle protein	-
BACIH_RS07860 (BACIH_1600)	rpsP	1535713..1535985 (+)	273	WP_003154296.1	30S ribosomal protein S16	-
BACIH_RS07865 (BACIH_1601)	-	1535985..1536230 (+)	246	WP_007611392.1	KH domain-containing protein	-
BACIH_RS07870 (BACIH_1602)	-	1536322..1536708 (+)	387	WP_003154291.1	YlqD family protein	-
BACIH_RS07875 (BACIH_1603)	rimM	1536713..1537237 (+)	525	WP_003154290.1	ribosome maturation factor RimM	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36168.34 Da
• Isoelectric Point: 4.9352

>NTDB_id=342261 BACIH_RS07840 WP_012117545.1 1531955..1532944(+) (pilA) [Bacillus amyloliquefaciens strain V167]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFSNKVNELVSRYRKVDEDFFEELEEVLIGADVGFTTVMELIDELKKEV
KLKNIQDPQEVQSVISEKLVDIYNSGDEQISELNIQDERLNVILLVGVNGVGKTTTIGKLAHKLKNEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQAAGSDPAAVIYDAVHAAKARGADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQRFDAESYVYGLF
SDLVEKAED

Nucleotide

• Length: 990 bp

>NTDB_id=342261 BACIH_RS07840 WP_012117545.1 1531955..1532944(+) (pilA) [Bacillus amyloliquefaciens strain V167]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACGAAACAGACAGACTCCGTCTCTGAAAAATTTAAAGATGGTCTTGA
AAAAACAAGAAATTCCTTCTCAAATAAAGTGAACGAACTCGTCTCCCGCTACCGGAAAGTCGACGAGGATTTCTTCGAGG
AGCTTGAGGAAGTGCTGATTGGCGCTGACGTAGGTTTTACAACCGTTATGGAGCTGATTGATGAGCTCAAAAAAGAAGTG
AAGCTGAAAAACATCCAAGATCCTCAGGAAGTGCAGTCCGTCATTTCTGAAAAGCTTGTCGACATTTATAACAGCGGCGA
CGAACAGATTTCTGAATTAAATATTCAGGACGAGCGCCTGAACGTTATTCTGCTTGTCGGGGTAAACGGCGTCGGAAAAA
CGACAACGATCGGAAAACTTGCCCATAAGCTGAAGAATGAAGGCAAATCCGTTGTGCTCGCTGCCGGAGACACTTTCAGA
GCCGGGGCGATTGAACAGCTTGAAGTGTGGGGGGAGCGTACAGGCGTGCCTGTCATTAAACAGGCTGCCGGAAGCGACCC
TGCTGCGGTTATTTATGATGCCGTACACGCGGCTAAAGCAAGAGGAGCGGATGTGCTGATTTGTGATACGGCCGGCCGCC
TTCAGAATAAAGTAAACTTAATGAAAGAGCTTGAAAAAGTCAAACGTGTCATCGAACGCGAAGTGCCTGAGGCTCCGCAT
GAGGTTCTCCTTGCGCTGGACGCGACGACAGGCCAAAATGCGATGGCGCAGGCGAAAGAATTCTCCAAAGCGACGAATGT
GACGGGTATCGCGTTAACGAAGCTTGACGGCACCGCTAAAGGCGGTATCGTCTTGGCGATCCGCAATGAGCTTCACATCC
CCGTGAAACTGGTCGGCCTCGGAGAAAAGGTTGACGATCTCCAGCGGTTTGATGCAGAATCTTACGTATACGGTCTCTTT
TCTGATTTAGTTGAAAAAGCCGAGGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (33-99)

3D structure

• AlphaFold DB: A7Z4L5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	45.333	91.185	0.413