Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F7D05_RS03150 Genome accession   NZ_CP044312
Coordinates   646730..647170 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain RM13745     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641730..652170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7D05_RS03130 (F7D05_03195) coaE 642220..642840 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  F7D05_RS26445 - 642865..642909 (+) 45 WP_120795372.1 protein YacM -
  F7D05_RS03135 (F7D05_03200) guaC 643065..644108 (+) 1044 WP_001217338.1 GMP reductase -
  F7D05_RS03140 (F7D05_03205) hofC 644143..645345 (-) 1203 WP_050938953.1 protein transport protein HofC -
  F7D05_RS03145 (F7D05_03210) pilB 645335..646720 (-) 1386 WP_050938954.1 type II secretion system protein GspE Machinery gene
  F7D05_RS03150 (F7D05_03215) pilA 646730..647170 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  F7D05_RS03155 (F7D05_03220) nadC 647373..648266 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  F7D05_RS03160 (F7D05_03225) ampD 648354..648905 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F7D05_RS03165 (F7D05_03230) ampE 648902..649756 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  F7D05_RS03170 (F7D05_03235) aroP 649799..651172 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=341835 F7D05_RS03150 WP_000360902.1 646730..647170(-) (pilA) [Escherichia coli strain RM13745]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=341835 F7D05_RS03150 WP_000360902.1 646730..647170(-) (pilA) [Escherichia coli strain RM13745]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377