Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETL60_RS02020 Genome accession   NZ_CP035414
Coordinates   369407..369856 (-) Length   149 a.a.
NCBI ID   WP_129092925.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103637     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364407..374856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETL60_RS01980 (ETL60_01980) - 364735..364931 (+) 197 Protein_340 hypothetical protein -
  ETL60_RS01985 (ETL60_01985) yckC 364950..365399 (+) 450 WP_087614278.1 RDD family protein -
  ETL60_RS01990 (ETL60_01990) yckD 365481..365813 (+) 333 WP_003246247.1 YckD family protein -
  ETL60_RS01995 (ETL60_01995) - 365941..366363 (+) 423 WP_198297576.1 MarR family transcriptional regulator -
  ETL60_RS02000 (ETL60_02000) - 366351..366692 (+) 342 WP_087614281.1 DUF3147 family protein -
  ETL60_RS02005 (ETL60_02005) - 366711..367439 (+) 729 WP_087614282.1 AAA family ATPase -
  ETL60_RS02010 (ETL60_02010) bglC 367512..368945 (+) 1434 WP_087614284.1 6-phospho-beta-glucosidase -
  ETL60_RS02015 (ETL60_02015) nin/comJ 368982..369380 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  ETL60_RS02020 (ETL60_02020) nucA/comI 369407..369856 (-) 450 WP_129092925.1 DNA-entry nuclease Machinery gene
  ETL60_RS02025 (ETL60_02025) tlpC 370024..371745 (-) 1722 WP_087614285.1 methyl-accepting chemotaxis protein TlpC -
  ETL60_RS02030 (ETL60_02030) hxlB 371856..372413 (-) 558 WP_046160042.1 6-phospho-3-hexuloisomerase -
  ETL60_RS02035 (ETL60_02035) hxlA 372419..373051 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  ETL60_RS02040 (ETL60_02040) hxlR 373283..373645 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=340843 ETL60_RS02020 WP_129092925.1 369407..369856(-) (nucA/comI) [Bacillus subtilis strain SRCM103637]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETITFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=340843 ETL60_RS02020 WP_129092925.1 369407..369856(-) (nucA/comI) [Bacillus subtilis strain SRCM103637]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCACCTTTCCTTCTGACCGTT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment