Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETK69_RS02215 Genome accession   NZ_CP035410
Coordinates   396819..397256 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM103616     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 391819..402256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK69_RS02190 (ETK69_02190) - 392840..393976 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  ETK69_RS02195 (ETK69_02195) - 393990..394424 (+) 435 WP_061861834.1 RDD family protein -
  ETK69_RS02200 (ETK69_02200) - 394496..394819 (+) 324 WP_007409357.1 YckD family protein -
  ETK69_RS02205 (ETK69_02205) - 394923..396359 (+) 1437 WP_061861835.1 family 1 glycosylhydrolase -
  ETK69_RS02210 (ETK69_02210) nin/comJ 396400..396798 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  ETK69_RS02215 (ETK69_02215) nucA/comI 396819..397256 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ETK69_RS02220 (ETK69_02220) hxlB 397587..398144 (-) 558 WP_065180943.1 6-phospho-3-hexuloisomerase -
  ETK69_RS02225 (ETK69_02225) hxlA 398141..398776 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  ETK69_RS02230 (ETK69_02230) - 399008..399370 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=340607 ETK69_RS02215 WP_003156588.1 396819..397256(-) (nucA/comI) [Bacillus velezensis strain SRCM103616]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=340607 ETK69_RS02215 WP_003156588.1 396819..397256(-) (nucA/comI) [Bacillus velezensis strain SRCM103616]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment