Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ES965_RS01975 Genome accession   NZ_CP035391
Coordinates   363336..363785 (-) Length   149 a.a.
NCBI ID   WP_124060108.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103689     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 358336..368785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES965_RS01940 (ES965_01940) yckA 358490..359170 (-) 681 WP_086343438.1 amino acid ABC transporter permease -
  ES965_RS01945 (ES965_01945) yckB 359180..360043 (-) 864 WP_086343439.1 transporter substrate-binding domain-containing protein -
  ES965_RS01950 (ES965_01950) - 360204..360400 (+) 197 Protein_335 hypothetical protein -
  ES965_RS01955 (ES965_01955) yckC 360419..360874 (+) 456 WP_064672016.1 RDD family protein -
  ES965_RS01960 (ES965_01960) yckD 360956..361288 (+) 333 WP_015252849.1 YckD family protein -
  ES965_RS01965 (ES965_01965) bglC 361441..362874 (+) 1434 WP_086343440.1 6-phospho-beta-glucosidase -
  ES965_RS01970 (ES965_01970) nin/comJ 362911..363309 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  ES965_RS01975 (ES965_01975) nucA/comI 363336..363785 (-) 450 WP_124060108.1 DNA-entry nuclease Machinery gene
  ES965_RS01980 (ES965_01980) tlpC 363953..365674 (-) 1722 WP_124060107.1 methyl-accepting chemotaxis protein TlpC -
  ES965_RS01985 (ES965_01985) hxlB 365785..366342 (-) 558 WP_024572182.1 6-phospho-3-hexuloisomerase -
  ES965_RS01990 (ES965_01990) hxlA 366348..366980 (-) 633 WP_024572183.1 3-hexulose-6-phosphate synthase -
  ES965_RS01995 (ES965_01995) hxlR 367214..367576 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16456.39 Da        Isoelectric Point: 4.5282

>NTDB_id=339589 ES965_RS01975 WP_124060108.1 363336..363785(-) (nucA/comI) [Bacillus subtilis strain SRCM103689]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETISFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=339589 ES965_RS01975 WP_124060108.1 363336..363785(-) (nucA/comI) [Bacillus subtilis strain SRCM103689]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCTCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment