Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ERO09_RS02470 Genome accession   NZ_CP035368
Coordinates   518370..518912 (+) Length   180 a.a.
NCBI ID   WP_149785854.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain LC_1315_18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 513370..523912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ERO09_RS02450 (ERO09_02440) - 514042..514938 (-) 897 WP_128787017.1 manganese/iron ABC transporter ATP-binding protein -
  ERO09_RS02455 (ERO09_02445) - 514938..515819 (-) 882 WP_075875732.1 metal ABC transporter substrate-binding protein -
  ERO09_RS02460 (ERO09_02450) - 515975..516850 (-) 876 WP_128787018.1 D-alanyl-D-alanine carboxypeptidase family protein -
  ERO09_RS02465 (ERO09_02455) - 517152..518300 (+) 1149 WP_049357003.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ERO09_RS02470 (ERO09_02460) pilF/pilF2 518370..518912 (+) 543 WP_149785854.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ERO09_RS02475 (ERO09_02465) - 518980..519966 (+) 987 WP_149785855.1 RodZ family helix-turn-helix domain-containing protein -
  ERO09_RS02480 (ERO09_02470) ispG 519985..521091 (+) 1107 WP_128787021.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ERO09_RS02485 (ERO09_02475) hisS 521101..522372 (+) 1272 WP_128787022.1 histidine--tRNA ligase -
  ERO09_RS02490 (ERO09_02480) - 522391..523005 (+) 615 WP_049356996.1 YfgM family protein -
  ERO09_RS02495 (ERO09_02485) - 523057..523500 (+) 444 WP_049356994.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20433.03 Da        Isoelectric Point: 5.9880

>NTDB_id=339346 ERO09_RS02470 WP_149785854.1 518370..518912(+) (pilF/pilF2) [Haemophilus parainfluenzae strain LC_1315_18]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDERYYLVHSALAHFYQ
LQGDSEKAKQAYLQAIHLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENIALCAFAGKQIDVYQQ
ALDKLRQVDPSRAEKLRTFK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=339346 ERO09_RS02470 WP_149785854.1 518370..518912(+) (pilF/pilF2) [Haemophilus parainfluenzae strain LC_1315_18]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCGGCTTGCGTTTCTCAGTCTTCAGT
CGATTTTAATAAACAACAAGCCGCAAAAGCGCGCGTTGAATTGGCATTAGGTTATCTGCAACAAGAGGATTTTGTTCAAG
CAAAACTGAATTTAGATAAAGCCTTTGAACATGATGAGCGCTATTATCTTGTACACTCAGCACTTGCACATTTTTATCAA
TTACAAGGCGATTCAGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTCACTTAGATGATAAGCAAGGCGACGTGTATAA
CAACTTTGGTGCTTTTTTATGTGGGCAAGGTGAGTTTGAACAAGCCTATTCACAATTTAATGCGGCACTTGCAGCACCGA
ATTATTATCATCAAGCCGATACTTACGAAAACATAGCACTTTGTGCTTTTGCTGGAAAACAAATCGATGTTTATCAGCAA
GCGTTGGATAAATTGCGCCAAGTTGATCCTTCTCGAGCGGAAAAGCTCCGTACGTTTAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

64.535

95.556

0.617
  pilF Glaesserella parasuis strain SC1401

49.444

100

0.494

Multiple sequence alignment