Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   EQW00_RS00400 Genome accession   NZ_CP035288
Coordinates   84876..86237 (-) Length   453 a.a.
NCBI ID   WP_001631036.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain ATCC 14990     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 47199..102102 84876..86237 within 0


Gene organization within MGE regions


Location: 47199..102102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQW00_RS00205 (EQW00_00205) - 47199..48878 (+) 1680 WP_083553768.1 recombinase family protein -
  EQW00_RS00210 (EQW00_00210) - 48967..49305 (+) 339 WP_032604336.1 SAUGI family uracil-DNA glycosylase inhibitor -
  EQW00_RS00220 (EQW00_00220) - 49399..49710 (+) 312 WP_032604337.1 DUF960 family protein -
  EQW00_RS00225 (EQW00_00225) - 49726..50229 (+) 504 WP_002437518.1 DUF1643 domain-containing protein -
  EQW00_RS00230 (EQW00_00230) - 50243..50464 (+) 222 WP_002437519.1 hypothetical protein -
  EQW00_RS00235 (EQW00_00235) - 50615..51181 (+) 567 WP_002437520.1 hypothetical protein -
  EQW00_RS00240 (EQW00_00240) - 51259..54378 (-) 3120 WP_083553769.1 type I restriction endonuclease subunit R -
  EQW00_RS00245 (EQW00_00245) - 54362..55564 (-) 1203 WP_083553770.1 restriction endonuclease subunit S -
  EQW00_RS00250 (EQW00_00250) - 55554..57068 (-) 1515 WP_083553771.1 class I SAM-dependent DNA methyltransferase -
  EQW00_RS00255 (EQW00_00255) - 57253..58227 (+) 975 WP_083553772.1 nucleotidyltransferase -
  EQW00_RS00260 (EQW00_00260) - 58280..58768 (+) 489 WP_244307884.1 hypothetical protein -
  EQW00_RS00265 (EQW00_00265) - 59153..59386 (-) 234 WP_002467980.1 hypothetical protein -
  EQW00_RS00270 (EQW00_00270) sesJ 59626..62859 (+) 3234 WP_083043952.1 MSCRAMM family cell wall-anchored protein SesJ -
  EQW00_RS00275 (EQW00_00275) gtfA 62948..64456 (+) 1509 WP_001551920.1 accessory Sec system glycosyltransferase GtfA -
  EQW00_RS00280 (EQW00_00280) gtfB 64449..65768 (+) 1320 WP_001551922.1 accessory Sec system glycosylation chaperone GtfB -
  EQW00_RS00285 (EQW00_00285) - 65949..66452 (-) 504 WP_001551924.1 DUF1643 domain-containing protein -
  EQW00_RS00290 (EQW00_00290) - 66467..66781 (-) 315 WP_001551925.1 DUF960 family protein -
  EQW00_RS00300 (EQW00_00300) - 66874..67215 (-) 342 WP_001551929.1 SAUGI family uracil-DNA glycosylase inhibitor -
  EQW00_RS00305 (EQW00_00305) ccrB 67739..69367 (-) 1629 WP_083553775.1 recombinase family protein Machinery gene
  EQW00_RS00310 (EQW00_00310) ccrA 69389..70738 (-) 1350 WP_001551932.1 recombinase family protein Machinery gene
  EQW00_RS00315 (EQW00_00315) - 70972..72765 (-) 1794 WP_002495148.1 DUF927 domain-containing protein -
  EQW00_RS00320 (EQW00_00320) - 72765..73061 (-) 297 WP_000221967.1 single-stranded DNA-binding protein -
  EQW00_RS00325 (EQW00_00325) - 73233..74816 (+) 1584 WP_001551935.1 hypothetical protein -
  EQW00_RS00330 (EQW00_00330) - 74888..75820 (+) 933 WP_000364405.1 hypothetical protein -
  EQW00_RS00335 (EQW00_00335) - 76246..76623 (+) 378 WP_000687965.1 hypothetical protein -
  EQW00_RS00340 (EQW00_00340) - 77092..77589 (+) 498 WP_000051479.1 hypothetical protein -
  EQW00_RS00345 (EQW00_00345) - 77845..77988 (+) 144 WP_001631028.1 restriction endonuclease subunit R -
  EQW00_RS00350 (EQW00_00350) - 77981..79183 (+) 1203 WP_001631029.1 restriction endonuclease subunit S -
  EQW00_RS00355 (EQW00_00355) - 79228..79444 (-) 217 Protein_65 IS5/IS1182 family transposase -
  EQW00_RS00360 (EQW00_00360) - 79506..80003 (-) 498 WP_001631030.1 GNAT family N-acetyltransferase -
  EQW00_RS00370 (EQW00_00370) - 80675..81085 (-) 411 WP_001631031.1 DUF2871 domain-containing protein -
  EQW00_RS12275 - 81248..81468 (-) 221 Protein_68 hypothetical protein -
  EQW00_RS00380 (EQW00_00380) - 81483..81986 (-) 504 WP_001631032.1 DUF1643 domain-containing protein -
  EQW00_RS00385 (EQW00_00385) - 82002..82313 (-) 312 WP_001631033.1 DUF960 domain-containing protein -
  EQW00_RS00390 (EQW00_00390) - 82400..82750 (-) 351 WP_001631034.1 SAUGI family uracil-DNA glycosylase inhibitor -
  EQW00_RS00395 (EQW00_00395) ccrB 83251..84879 (-) 1629 WP_001631035.1 recombinase family protein Machinery gene
  EQW00_RS00400 (EQW00_00400) ccrA 84876..86237 (-) 1362 WP_001631036.1 recombinase family protein Machinery gene
  EQW00_RS00405 (EQW00_00405) - 86424..87053 (-) 630 Protein_74 hypothetical protein -
  EQW00_RS12280 - 87062..87400 (+) 339 Protein_75 recombinase family protein -
  EQW00_RS00415 (EQW00_00415) - 87512..87871 (-) 360 WP_001631039.1 hypothetical protein -
  EQW00_RS00420 (EQW00_00420) - 88078..88404 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  EQW00_RS00430 (EQW00_00430) - 88734..90659 (+) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -
  EQW00_RS00435 (EQW00_00435) - 91782..92732 (-) 951 Protein_79 transposase -
  EQW00_RS00440 (EQW00_00440) - 92853..93422 (+) 570 WP_002470562.1 hypothetical protein -
  EQW00_RS00445 (EQW00_00445) - 93467..94624 (+) 1158 WP_002485721.1 hypothetical protein -
  EQW00_RS00450 (EQW00_00450) - 94807..95079 (+) 273 WP_002501902.1 hypothetical protein -
  EQW00_RS00455 (EQW00_00455) - 95109..95783 (+) 675 WP_134943505.1 IS6-like element IS257 family transposase -
  EQW00_RS00460 (EQW00_00460) lspA 95811..96263 (-) 453 WP_164071529.1 signal peptidase II -
  EQW00_RS00465 (EQW00_00465) - 96423..96824 (+) 402 WP_057504659.1 metalloregulator ArsR/SmtB family transcription factor -
  EQW00_RS00470 (EQW00_00470) - 97078..97845 (+) 768 WP_057504658.1 ZIP family metal transporter -
  EQW00_RS00475 (EQW00_00475) - 98102..100510 (+) 2409 WP_002485759.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 53316.45 Da        Isoelectric Point: 9.8596

>NTDB_id=338934 EQW00_RS00400 WP_001631036.1 84876..86237(-) (ccrA) [Staphylococcus epidermidis strain ATCC 14990]
MEEAIAYVRQSTLKQQSLATQKSLIMDTAKQYGWSNVAFYDDKKTGRHTKRSGYQKMVEIITSGKCKVLCCYRLNRLHRN
LKNAIQFFEICKKHHITIISVNDGYFDLSKEFDCFRLNILMSLAEMESNNISEQTKNGIREKAKQGKLITTHAPFGYRYR
KGCFVVQEEEAYTVKSVYRWYLQGLGYKKISQHLDSNPNLIPRKPYQVRNILLNPNYCGRVINKYGTFNDIVPPIIDIDT
FEEAQERRIHKQLNRSNSRNKLKRRIKCPYCQSTLTNLTIKKENYSLRYYVCPKNMNDAYRTCPFKSINAPELESEVLDT
CKKYFEEQSFHKRLNNTILKVLKQQQMKHKETHLTQEQLIEKLAQNRIDVKTFKRLSASCKSEENYSTYSTYQIDDKLKY
VIKNKITLQDIAPFIDCIKITQTKQLQGIYFKNSPLNIVEQSHLITPERNEVI

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=338934 EQW00_RS00400 WP_001631036.1 84876..86237(-) (ccrA) [Staphylococcus epidermidis strain ATCC 14990]
ATGGAAGAAGCGATTGCTTATGTACGTCAAAGTACACTCAAACAACAAAGCTTAGCTACTCAAAAATCGCTCATTATGGA
TACCGCTAAGCAATATGGATGGTCTAATGTGGCGTTTTATGATGATAAGAAAACAGGTCGACATACAAAACGTTCAGGTT
ATCAAAAAATGGTAGAAATAATTACGTCTGGAAAATGTAAAGTATTGTGTTGCTATCGCTTGAACCGACTACATCGTAAT
TTGAAAAATGCCATACAATTCTTTGAAATTTGTAAAAAGCATCACATCACAATCATCAGTGTTAACGATGGATATTTTGA
TTTGTCTAAAGAGTTTGATTGCTTTCGTTTGAACATACTTATGAGTCTAGCGGAAATGGAATCAAACAATATCAGCGAAC
AAACAAAAAATGGGATTAGAGAAAAAGCCAAACAAGGTAAGCTAATCACAACTCATGCTCCGTTTGGCTATCGTTATAGA
AAAGGTTGTTTTGTTGTACAAGAAGAAGAAGCGTATACAGTTAAATCGGTTTATCGCTGGTACTTACAAGGTCTAGGCTA
CAAAAAGATTTCCCAACATTTGGATAGTAATCCTAACCTTATTCCTCGTAAGCCCTATCAAGTTAGAAATATTCTCTTGA
ACCCTAACTATTGCGGTCGTGTCATCAATAAATATGGCACGTTTAATGATATCGTTCCACCTATTATTGATATTGATACA
TTTGAAGAAGCACAAGAGCGTCGTATTCATAAACAACTCAATCGATCCAATTCTAGAAATAAGCTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAGAACTATTCTCTACGTTACTATGTTTGTCCTA
AAAATATGAATGACGCTTATCGTACATGCCCATTTAAAAGTATCAATGCACCAGAACTTGAAAGTGAAGTGCTCGACACT
TGTAAAAAGTATTTTGAAGAACAATCGTTTCATAAACGATTGAATAACACAATACTCAAAGTCCTAAAACAGCAACAAAT
GAAGCATAAAGAAACCCATCTAACACAAGAACAACTTATCGAAAAGCTAGCTCAAAATCGTATTGATGTAAAAACGTTCA
AACGTTTATCTGCTAGTTGTAAAAGTGAAGAGAATTACTCTACTTATTCAACCTACCAAATCGATGACAAATTAAAATAT
GTCATAAAAAATAAAATAACTTTACAGGATATAGCACCTTTTATCGATTGCATCAAAATAACTCAAACTAAACAATTACA
AGGAATCTACTTTAAAAACAGTCCACTCAATATCGTTGAACAATCACATTTAATAACTCCTGAAAGGAATGAAGTAATAT
GA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(168-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

54.628

97.792

0.534
  ccrA Staphylococcus aureus N315

50.564

97.792

0.494

Multiple sequence alignment