Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   F1748_RS20175 Genome accession   NZ_CP043821
Coordinates   4056579..4057343 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain RM10740     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4051579..4062343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1748_RS20155 (F1748_20255) acpT 4052533..4053120 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  F1748_RS20160 (F1748_20260) nikA 4053231..4054805 (+) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  F1748_RS20165 (F1748_20265) nikB 4054805..4055749 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  F1748_RS20170 (F1748_20270) nikC 4055746..4056579 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  F1748_RS20175 (F1748_20275) amiE 4056579..4057343 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  F1748_RS20180 (F1748_20280) nikE 4057340..4058146 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  F1748_RS20185 (F1748_20285) nikR 4058152..4058553 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  F1748_RS20190 (F1748_20290) - 4058673..4059032 (-) 360 WP_238005030.1 type II toxin-antitoxin system HicB family antitoxin -
  F1748_RS20195 (F1748_20295) - 4059029..4059259 (-) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  F1748_RS20200 (F1748_20300) yhhJ 4059363..4060487 (-) 1125 WP_001314210.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=338933 F1748_RS20175 WP_001136236.1 4056579..4057343(+) (amiE) [Escherichia coli strain RM10740]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=338933 F1748_RS20175 WP_001136236.1 4056579..4057343(+) (amiE) [Escherichia coli strain RM10740]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment