Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EQH20_RS10005 Genome accession   NZ_CP035260
Coordinates   1967413..1968711 (-) Length   432 a.a.
NCBI ID   WP_000867605.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901925     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1962413..1973711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH20_RS09985 (EQH20_10520) rplI 1963568..1964020 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  EQH20_RS09990 (EQH20_10525) - 1964017..1965990 (-) 1974 WP_000715130.1 DHH family phosphoesterase -
  EQH20_RS09995 (EQH20_10530) hpf 1966126..1966674 (-) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EQH20_RS10000 (EQH20_10535) comFC/cflB 1966754..1967416 (-) 663 WP_000649974.1 ComF family protein Machinery gene
  EQH20_RS10005 (EQH20_10540) comFA/cflA 1967413..1968711 (-) 1299 WP_000867605.1 DEAD/DEAH box helicase Machinery gene
  EQH20_RS10010 (EQH20_10545) - 1968767..1969402 (+) 636 WP_000395730.1 YigZ family protein -
  EQH20_RS10015 (EQH20_10550) cysK 1969687..1970607 (+) 921 WP_000029866.1 cysteine synthase A -
  EQH20_RS10020 (EQH20_10560) - 1970846..1971220 (-) 375 Protein_1952 IS66 family transposase -
  EQH20_RS10025 (EQH20_10565) - 1971193..1971501 (+) 309 Protein_1953 transposase -
  EQH20_RS10030 (EQH20_10570) tnpB 1971692..1971979 (-) 288 WP_000586646.1 IS66 family insertion sequence element accessory protein TnpB -
  EQH20_RS10035 (EQH20_10575) tsf 1972225..1973265 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49620.59 Da        Isoelectric Point: 8.4382

>NTDB_id=338350 EQH20_RS10005 WP_000867605.1 1967413..1968711(-) (comFA/cflA) [Streptococcus pneumoniae strain TVO_1901925]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=338350 EQH20_RS10005 WP_000867605.1 1967413..1968711(-) (comFA/cflA) [Streptococcus pneumoniae strain TVO_1901925]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCAATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae TIGR4

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment