Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQH20_RS00135 Genome accession   NZ_CP035260
Coordinates   24866..26227 (+) Length   453 a.a.
NCBI ID   WP_078152438.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901925     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 19866..31227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH20_RS00100 (EQH20_00100) - 20246..21019 (-) 774 Protein_14 transposase -
  EQH20_RS00105 (EQH20_00105) comW 21284..21520 (+) 237 WP_000939546.1 sigma(X)-activator ComW Regulator
  EQH20_RS00110 (EQH20_00110) - 21751..23037 (+) 1287 WP_001818679.1 adenylosuccinate synthase -
  EQH20_RS00115 (EQH20_00115) tadA 23238..23705 (+) 468 WP_000291874.1 tRNA adenosine(34) deaminase TadA -
  EQH20_RS00125 (EQH20_00125) - 23892..24335 (+) 444 WP_000701992.1 dUTP diphosphatase -
  EQH20_RS00130 (EQH20_00130) - 24337..24852 (+) 516 WP_001823418.1 histidine phosphatase family protein -
  EQH20_RS00135 (EQH20_00135) radA 24866..26227 (+) 1362 WP_078152438.1 DNA repair protein RadA Machinery gene
  EQH20_RS00140 (EQH20_00140) - 26300..26797 (+) 498 WP_001809263.1 carbonic anhydrase -
  EQH20_RS00145 (EQH20_00150) - 26822..27636 (+) 815 Protein_22 PrsW family intramembrane metalloprotease -
  EQH20_RS00150 (EQH20_00155) - 27781..28749 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  EQH20_RS00155 (EQH20_00160) - 28883..29164 (-) 282 Protein_24 ISL3 family transposase -
  EQH20_RS00160 - 29291..30201 (-) 911 Protein_25 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49447.92 Da        Isoelectric Point: 6.5137

>NTDB_id=338282 EQH20_RS00135 WP_078152438.1 24866..26227(+) (radA) [Streptococcus pneumoniae strain TVO_1901925]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLQQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=338282 EQH20_RS00135 WP_078152438.1 24866..26227(+) (radA) [Streptococcus pneumoniae strain TVO_1901925]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCAACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTTATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTAGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAACCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA Bacillus subtilis subsp. subtilis str. 168

62.914

100

0.629

Multiple sequence alignment