Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FZN31_RS04225 Genome accession   NZ_CP043414
Coordinates   887254..888063 (+) Length   269 a.a.
NCBI ID   WP_105287678.1    Uniprot ID   -
Organism   Escherichia coli strain EC42405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 882254..893063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZN31_RS04220 (FZN31_04330) sslE 882395..886957 (+) 4563 WP_105287679.1 lipoprotein metalloprotease SslE -
  FZN31_RS04225 (FZN31_04345) pilD 887254..888063 (+) 810 WP_105287678.1 prepilin peptidase PppA Machinery gene
  FZN31_RS04230 (FZN31_04350) gspS2 888129..888539 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  FZN31_RS04235 (FZN31_04355) gspC 888555..889508 (+) 954 WP_105287677.1 type II secretion system protein GspC -
  FZN31_RS04240 (FZN31_04360) gspD 889538..891598 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29449.07 Da        Isoelectric Point: 8.3801

>NTDB_id=336997 FZN31_RS04225 WP_105287678.1 887254..888063(+) (pilD) [Escherichia coli strain EC42405]
MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMIRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWTQQSPLTLQDSVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=336997 FZN31_RS04225 WP_105287678.1 887254..888063(+) (pilD) [Escherichia coli strain EC42405]
ATGCTTTTTGATGTTTTTCAGCAATACCCTGCGGCGATGCCCATACTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTATCCCATCATGATTCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTGGCGCTGCCGCGTTCGCACTGTCCGCATTGTCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTGCTCTCCTGGTTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTCGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTTATTGCCGCGAGCATCATCGACCTTGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGACGCAACAGAGTCCGTTAACGCTACAAGACTCAGTTACCGGCGTCCTGGTGGGATTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTTGCCG
CGTTAGGTGGCTGGGTGGGCGCGTTGTCGCTGCCCAATGTGGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

41.697

100

0.42
  pilD Neisseria gonorrhoeae MS11

38.911

95.539

0.372
  pilD Acinetobacter nosocomialis M2

37.736

98.513

0.372
  pilD Acinetobacter baumannii D1279779

37.358

98.513

0.368

Multiple sequence alignment