Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EQZ20_RS14735 Genome accession   NZ_CP035232
Coordinates   2858618..2859685 (-) Length   355 a.a.
NCBI ID   WP_046129974.1    Uniprot ID   -
Organism   Bacillus glycinifermentans strain SRCM103574     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2853618..2864685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQZ20_RS14690 (EQZ20_14690) tapA 2854075..2854800 (-) 726 WP_046129966.1 amyloid fiber anchoring/assembly protein TapA -
  EQZ20_RS14695 (EQZ20_14695) - 2855063..2855386 (+) 324 WP_046129967.1 DUF3889 domain-containing protein -
  EQZ20_RS14700 (EQZ20_14700) - 2855471..2855653 (-) 183 WP_046129968.1 YqzE family protein -
  EQZ20_RS14705 (EQZ20_14705) comGG 2855735..2856100 (-) 366 WP_046129969.1 competence type IV pilus minor pilin ComGG -
  EQZ20_RS14710 (EQZ20_14710) comGF 2856112..2856597 (-) 486 WP_082094061.1 competence type IV pilus minor pilin ComGF -
  EQZ20_RS14715 (EQZ20_14715) comGE 2856512..2856859 (-) 348 WP_046129970.1 competence type IV pilus minor pilin ComGE -
  EQZ20_RS14720 (EQZ20_14720) comGD 2856843..2857286 (-) 444 WP_046129971.1 competence type IV pilus minor pilin ComGD -
  EQZ20_RS14725 (EQZ20_14725) comGC 2857286..2857579 (-) 294 WP_046129972.1 competence type IV pilus major pilin ComGC Machinery gene
  EQZ20_RS14730 (EQZ20_14730) comGB 2857594..2858631 (-) 1038 WP_046129973.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQZ20_RS14735 (EQZ20_14735) comGA 2858618..2859685 (-) 1068 WP_046129974.1 competence type IV pilus ATPase ComGA Machinery gene
  EQZ20_RS14740 (EQZ20_14740) - 2859842..2860681 (-) 840 WP_046129975.1 STAS domain-containing protein -
  EQZ20_RS14750 (EQZ20_14750) - 2860894..2861274 (-) 381 WP_046129976.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  EQZ20_RS14755 (EQZ20_14755) - 2861478..2861723 (+) 246 WP_046129977.1 DUF2626 domain-containing protein -
  EQZ20_RS14760 (EQZ20_14760) - 2861772..2862410 (-) 639 WP_046129978.1 MBL fold metallo-hydrolase -
  EQZ20_RS14765 (EQZ20_14765) - 2862551..2862724 (+) 174 WP_046129979.1 DUF2759 domain-containing protein -
  EQZ20_RS14770 (EQZ20_14770) - 2862795..2863109 (-) 315 WP_046129980.1 MTH1187 family thiamine-binding protein -
  EQZ20_RS14775 (EQZ20_14775) - 2863126..2864229 (-) 1104 WP_046129981.1 hypothetical protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39803.27 Da        Isoelectric Point: 9.2825

>NTDB_id=336535 EQZ20_RS14735 WP_046129974.1 2858618..2859685(-) (comGA) [Bacillus glycinifermentans strain SRCM103574]
MYAIESLSGKLIEEACAMRASDIHIVPGEKEAVIRFRIDDELFQKGRLTRMECSRLISHFKFLSSMDIGERRQPQSGALT
IKVNNQPVHLRMSTLPTIYDESLVIRVLPQASAPPLRSLSLFPNATAKLLSFLKHSHGLLIFTGPTGSGKTTTLYSLIEY
AKQHFNRNIITLEDPVESRSEHVLQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAKIAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGIHMTEIEQTLVAISAQRLVNLVCPLCGERCSLYCRMSGNGRRVSVFELLYGKSLNLCIKEAKGAY
VNSRFETLRKLIRKGIALGYLPEETYNRWVHHETE

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=336535 EQZ20_RS14735 WP_046129974.1 2858618..2859685(-) (comGA) [Bacillus glycinifermentans strain SRCM103574]
GTGTACGCGATTGAATCATTAAGCGGGAAATTGATCGAAGAGGCGTGTGCGATGAGAGCTTCTGATATTCATATCGTTCC
GGGGGAAAAAGAGGCGGTTATCCGCTTTAGAATTGATGATGAACTATTTCAAAAGGGCAGACTGACGAGAATGGAGTGCT
CAAGGCTCATTTCTCATTTTAAATTTCTTTCTTCAATGGATATCGGGGAGCGAAGGCAGCCGCAAAGCGGTGCTTTAACC
ATTAAAGTGAACAATCAGCCCGTTCACTTGAGAATGTCGACTTTGCCTACCATATACGACGAAAGCCTGGTTATTCGCGT
ATTGCCGCAGGCAAGCGCCCCGCCGCTCAGAAGCCTGTCTTTGTTTCCAAACGCAACGGCAAAGCTGCTGTCTTTTCTGA
AACATTCCCACGGTCTGCTGATCTTCACAGGTCCAACCGGTTCGGGAAAAACGACGACCCTGTACTCGCTGATCGAGTAT
GCCAAACAGCATTTCAACCGCAATATTATCACGCTGGAGGACCCGGTTGAATCCAGAAGCGAGCATGTTCTTCAAGTACA
GGTGAATGAAAAAGCGGGCATGACGTATTCAGCAGGTTTAAAGGCTGTTCTCCGTCATGATCCCGACATGATCATCCTTG
GAGAAATCCGCGATGCCGAAACAGCCAAAATCGCCGTCAGAGCTGCGCTGACGGGACATCTTGTATTATCTAGCATGCAT
GCGAAAAACGCAAAAGGAGCTATATACCGGCTGCTTGAGTTTGGCATTCATATGACCGAAATTGAGCAGACGCTTGTCGC
TATAAGCGCACAGCGTCTCGTCAACCTTGTTTGTCCGTTATGCGGGGAGCGGTGTTCTTTGTATTGCAGGATGTCGGGGA
ACGGAAGAAGAGTGAGCGTTTTTGAGCTTTTGTACGGGAAGAGTCTGAACCTGTGCATAAAAGAGGCAAAAGGCGCATAC
GTGAACAGCCGTTTTGAAACGCTGAGAAAATTGATTCGAAAAGGGATAGCGCTCGGCTATCTCCCGGAGGAAACTTATAA
CAGATGGGTGCATCATGAGACTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

69.944

100

0.701
  pilB Glaesserella parasuis strain SC1401

39.437

100

0.394

Multiple sequence alignment