Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EQZ20_RS06255 Genome accession   NZ_CP035232
Coordinates   1244036..1247728 (+) Length   1230 a.a.
NCBI ID   WP_046131291.1    Uniprot ID   A0AAE2JVM9
Organism   Bacillus glycinifermentans strain SRCM103574     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1239036..1252728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQZ20_RS06245 (EQZ20_06245) modB 1239784..1240440 (+) 657 WP_046131293.1 molybdate ABC transporter permease subunit -
  EQZ20_RS06250 (EQZ20_06250) addB 1240549..1244049 (+) 3501 WP_046131292.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EQZ20_RS06255 (EQZ20_06255) addA 1244036..1247728 (+) 3693 WP_046131291.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EQZ20_RS06260 (EQZ20_06260) sbcD 1247796..1248980 (+) 1185 WP_046131290.1 exonuclease subunit SbcD -
  EQZ20_RS06265 (EQZ20_06265) sbcC 1248977..1252369 (+) 3393 WP_046131289.1 exonuclease subunit SbcC -
  EQZ20_RS06270 (EQZ20_06270) - 1252428..1252646 (-) 219 WP_046131288.1 spore germination protein -

Sequence

Protein


Download         Length: 1230 a.a.        Molecular weight: 141344.16 Da        Isoelectric Point: 5.4779

>NTDB_id=336511 EQZ20_RS06255 WP_046131291.1 1244036..1247728(+) (addA) [Bacillus glycinifermentans strain SRCM103574]
METAKPKNSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIGEA
LEKKLAENPGSLHLRRQLSLLNRASISTLHSFCLQVVRKYYYMIDVDPAFRIADQTEGELLGDEVLDELFEEEYQKGDPA
FFELVDRYTTDRHDLDLQYLIKRVYEFSRSHPDPEAWLEKFMNLYDADPGAKIESLPFYSYIREDIALVLNSVREKLTRA
LELTKRPGGPAPRAENFLDDLAQIDKLLEHQDDFEALYELVPAVSFQRAKPCKGDDFDPSLIDEATDLRNSAKKMLEKLK
GDYFTRTPEQHLESLKEMKPVIRTLVQLVAEFGRRYRAAKKEKAIVDFSDLEHDCLAILTKKNEGGTTEPSEAAAYYRHL
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGAGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVVYDEQAELKLGASYPPNESTKTEVLIIENGEDAADEEAEELETVQLEARAVAGRVREL
ISGKFQVYDGKTKTTRNIQYRDIVILLRSMPWAPQFMDEFKQQGIPVYANLSTGYFEATEVAVTLSLLKVIDNAYQDIPL
ASVLRSPIVGLDENELSLIRIRDKKAPFYEALKAYMASADRDDPLYEKLARFDGFLKKWRAYAKNHSVAELIWEVYRDTK
YIDYVGGMPGGKQRQANLRALYDRARSYESTSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFLVG
TAKDKDKLLAKWRTSASHAEWLLPDFERFQAKSYLDFIGPALMRHRDMADGDLNLPNDDIRQHPSRFTISWISAGELQAE
DRAQTEEEKHERLSLVKKGLPIDGAFDYEKEVRERLTWSYPFRDASQVRTKQSVSEIKRQKEYEDEYGDRSLIRPANETL
LFRRPSFMMAKGLTAAERGTAMHTVMQHLPLTRVPEKDELGRLLDMLIEKELLTEEQRAAIEEDDILAFFDTDIGGKLLG
ARRVEREVPFNMTLPAREVYPGFKTADDPVLIQGIIDCLFETDDGFYLLDYKTDRIHGKFQNGFEGAEPILRKRYETQIN
LYARAVETMLKTPLRGKALYFFDGGHVILF

Nucleotide


Download         Length: 3693 bp        

>NTDB_id=336511 EQZ20_RS06255 WP_046131291.1 1244036..1247728(+) (addA) [Bacillus glycinifermentans strain SRCM103574]
ATGGAAACGGCTAAGCCGAAAAACAGCACATGGACGGACGACCAATGGAAAGCGATCGTCTCATCCGGCCGGGATATTTT
GGTCGCCGCTGCGGCGGGCTCCGGAAAAACGGCCGTCCTTGTCGAGCGGATCATCCGAAAGATTACGGCAGAGGAAAATC
CGATCGATGTCGACCGGCTGCTCGTCGTCACCTTTACGAATGCATCGGCGGCGGAGATGAAACACCGGATCGGTGAAGCG
CTTGAGAAAAAGCTCGCCGAAAACCCCGGCTCCCTTCATTTGCGCAGGCAGCTTTCCTTGCTGAACAGGGCGAGCATTTC
GACATTGCATTCTTTTTGTCTTCAGGTCGTCCGCAAGTATTATTATATGATCGACGTTGATCCGGCTTTTCGGATCGCCG
ATCAGACAGAAGGCGAGCTGCTCGGCGATGAGGTCCTTGACGAGCTGTTTGAAGAAGAATATCAAAAAGGCGACCCGGCT
TTTTTTGAGCTGGTGGACAGATATACGACAGACCGCCACGATCTCGATTTGCAGTACCTTATCAAACGGGTTTACGAGTT
TTCCCGCTCACATCCCGACCCGGAAGCGTGGCTTGAAAAATTTATGAACCTGTACGACGCAGATCCCGGCGCGAAGATCG
AGTCGCTGCCGTTTTATTCCTACATCAGGGAAGATATCGCGCTTGTGTTGAACAGTGTGCGGGAAAAGCTGACCCGCGCG
CTTGAGCTGACAAAACGGCCGGGGGGGCCGGCTCCGCGCGCTGAAAACTTTCTCGACGATTTGGCGCAGATCGACAAGCT
GCTTGAGCATCAGGATGATTTTGAGGCCCTTTATGAGCTTGTGCCGGCTGTTTCGTTTCAGCGGGCCAAACCGTGCAAAG
GGGATGATTTCGATCCGTCTTTAATCGACGAAGCGACAGACCTTCGGAACAGTGCAAAAAAAATGCTTGAAAAGCTGAAA
GGCGACTATTTCACCCGAACGCCGGAGCAGCATTTGGAGAGTTTGAAGGAGATGAAGCCCGTCATCAGGACGCTTGTCCA
GCTCGTCGCAGAGTTCGGCAGACGCTATAGAGCGGCGAAGAAAGAAAAAGCCATCGTCGACTTCTCCGATCTTGAGCACG
ACTGCCTGGCGATTTTGACAAAGAAAAACGAAGGCGGGACAACGGAGCCGAGCGAAGCTGCGGCCTATTACCGGCACTTG
TTCCACGAAGTGCTCGTCGATGAGTATCAGGATACAAACCTTGTACAGGAGGCGATTTTAAAGCTTGTCGCCAAGGAGGA
GCACGAAGGAAACCTTTTTATGGTGGGCGATGTCAAGCAGTCGATCTACCGTTTCCGGCTGGCTGAGCCGCTTTTGTTTC
TTTCGAAATACAAGCGGTTCACAGATGACGGTTCAGGCGCCGGACAGAAAATCGATTTGAACAAAAACTTCAGAAGCCGG
TCCGACATTTTGGATAGCACCAATTTTTTGTTTAAACAGCTGATGGGGGAGAAGGTCGGCGAGGTTGTCTATGACGAACA
AGCCGAATTAAAGCTCGGCGCATCGTATCCTCCCAATGAGAGCACAAAAACAGAGGTGCTGATCATTGAAAATGGGGAAG
ATGCGGCTGATGAAGAGGCAGAAGAGCTTGAAACGGTTCAGCTTGAGGCAAGGGCTGTCGCCGGACGGGTCAGAGAGCTG
ATTTCCGGCAAATTTCAAGTGTATGACGGCAAAACGAAAACGACAAGAAACATCCAGTACCGCGACATCGTGATTTTGCT
GCGCTCCATGCCGTGGGCGCCGCAATTCATGGACGAGTTCAAGCAGCAGGGGATTCCGGTATACGCGAATCTGTCAACCG
GATATTTCGAAGCGACAGAAGTAGCCGTTACGCTTTCACTGCTTAAAGTCATCGACAATGCCTACCAGGATATTCCGCTT
GCATCCGTCCTCAGGTCACCGATTGTCGGCCTTGATGAAAACGAACTGTCCCTGATCAGAATCCGGGATAAAAAAGCGCC
GTTCTATGAAGCGCTGAAGGCGTATATGGCGTCAGCGGACAGGGATGATCCGCTTTATGAAAAGCTTGCCCGCTTTGACG
GGTTCCTGAAGAAGTGGAGGGCATACGCAAAAAACCATTCCGTGGCAGAGCTGATATGGGAAGTTTACCGCGATACGAAA
TATATCGATTATGTCGGCGGAATGCCGGGCGGAAAGCAGCGGCAAGCCAATTTGCGCGCGCTCTATGACAGGGCGAGATC
GTACGAATCGACCTCCTTCCGCGGATTGTTCCGCTTTTTGCGATTTATTGAACGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCGCGGGCTTTGAGCGAACAGGAAGACGTCGTCCGCCTGATGACGATCCATTCGAGCAAAGGCCTTGAATTTCCC
GTCGTATTCACGGCGGGCCTCGGCCGGAATTTCAATATGATGGATCTCAACAAATCGTATCTGCTTGATAAAGAGCTCGG
GTTCGGGACGAAATTTATCCATCCGAAATGGCGCATCAGCTATCCGACGCTGCCGCTTGTGGCGATGAAGAAAAAAATGC
GGCGCGAGCTTTTATCAGAAGAGCTCCGCGTCTTGTATGTCGCTCTCACCCGTGCAAAGGAAAAACTCTTTTTGGTCGGA
ACGGCGAAAGACAAGGACAAGCTTTTGGCAAAATGGCGGACCTCCGCCTCCCATGCCGAATGGCTGCTTCCTGATTTCGA
ACGCTTTCAGGCGAAATCGTATCTCGATTTTATCGGCCCAGCTTTAATGCGGCACAGGGATATGGCAGACGGTGATCTGA
ATTTGCCGAATGATGACATCCGTCAGCATCCGTCCCGTTTTACGATCAGCTGGATATCAGCAGGAGAGCTGCAGGCCGAA
GATAGAGCGCAGACTGAAGAAGAAAAACACGAACGGCTGTCACTGGTAAAAAAAGGGCTGCCGATCGATGGGGCTTTCGA
TTATGAAAAAGAAGTCCGCGAACGGCTGACATGGTCGTATCCTTTCCGGGATGCGTCACAGGTCCGCACAAAACAGTCTG
TCTCCGAAATCAAGAGACAGAAGGAGTACGAGGATGAGTACGGCGACCGTTCATTGATTCGTCCGGCAAATGAAACTTTG
CTGTTCAGAAGACCCTCTTTCATGATGGCAAAAGGGCTGACCGCCGCTGAGCGGGGAACGGCAATGCATACAGTCATGCA
GCATCTTCCGCTCACTCGCGTCCCCGAAAAGGATGAGCTCGGCCGTCTGCTGGACATGCTTATCGAAAAAGAGCTTCTAA
CGGAAGAGCAACGCGCAGCTATCGAAGAAGATGACATTCTCGCGTTTTTTGATACGGATATAGGCGGAAAACTGCTTGGC
GCCCGCCGGGTTGAGCGGGAAGTGCCCTTCAACATGACGCTGCCTGCACGAGAGGTTTATCCAGGCTTCAAGACTGCCGA
TGACCCCGTCTTAATTCAAGGGATTATCGACTGCTTGTTCGAAACCGATGACGGCTTTTATTTGCTTGACTACAAAACCG
ACCGGATACACGGCAAATTTCAAAACGGCTTTGAAGGAGCGGAGCCGATTTTACGAAAACGCTACGAGACGCAGATCAAC
CTTTACGCCCGGGCTGTCGAAACGATGCTTAAGACGCCTCTGAGAGGAAAGGCTCTTTACTTTTTCGACGGCGGGCACGT
GATCCTGTTTTGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(471-880)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-460)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1051-1222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

73.74

100

0.737

Multiple sequence alignment