Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EQZ20_RS06250 Genome accession   NZ_CP035232
Coordinates   1240549..1244049 (+) Length   1166 a.a.
NCBI ID   WP_046131292.1    Uniprot ID   A0AAE2EN29
Organism   Bacillus glycinifermentans strain SRCM103574     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1235549..1249049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQZ20_RS06225 (EQZ20_06225) - 1236221..1237591 (-) 1371 WP_046131295.1 HAMP domain-containing sensor histidine kinase -
  EQZ20_RS06230 (EQZ20_06230) - 1237572..1238264 (-) 693 WP_082094113.1 response regulator transcription factor -
  EQZ20_RS06235 (EQZ20_06235) - 1238349..1238801 (-) 453 WP_046131323.1 ferritin-like domain-containing protein -
  EQZ20_RS06240 (EQZ20_06240) modA 1238984..1239775 (+) 792 WP_046131294.1 molybdate ABC transporter substrate-binding protein -
  EQZ20_RS06245 (EQZ20_06245) modB 1239784..1240440 (+) 657 WP_046131293.1 molybdate ABC transporter permease subunit -
  EQZ20_RS06250 (EQZ20_06250) addB 1240549..1244049 (+) 3501 WP_046131292.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EQZ20_RS06255 (EQZ20_06255) addA 1244036..1247728 (+) 3693 WP_046131291.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EQZ20_RS06260 (EQZ20_06260) sbcD 1247796..1248980 (+) 1185 WP_046131290.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134038.68 Da        Isoelectric Point: 5.3215

>NTDB_id=336510 EQZ20_RS06250 WP_046131292.1 1240549..1244049(+) (addB) [Bacillus glycinifermentans strain SRCM103574]
MGAVFLSGRSGSGKTTFILDDIREKLRHDPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRILQHTG
GMNRPFLTGTGIQMLLRKLIEEHKSEFKVYQNASDKSGFTEQVERMLTEFKRHCLPPEGIREIMQGSGASEYEEERILSD
KLHDLYILYSRMEEALEHQYVQSEDYLTLLAEQIPHAAEIRDASIYVDGFHQFTPQEMRVLEQLMAHAREITFSLTVDEP
FAHHAPHDLHLFRMTGKAYFDLCQKARELGLDLSEVRMEGAKRHSHAPELEHLERQFDERPAKPYTGTSESFCLMQAANR
RTEIEGIAREIHSLVHSGRFRLKDIAVIARNLEDYKDTIKEVFTDYELSFFIDGKESMQNHPLIELIRSTLDIIKSNWRY
EAVFRCIKTELLFPMDEPRERVRDQIDQLENYCIAYGIKGERWTSKDPFTYRRYASLDDDFAKTDREIETENMLNDLRSW
IVPPVHRLQKRLKKAGSVREMAEAVYLYLEEADVPMKLEQERQLAEESGRIAESRQHDQVWDAVIQLLDEFVEMMGDERI
SFSLFQQMVETGLESLTFSLIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLTDDDREWLRRNGVQLA
ATGREQLLDEHFLIYMALSSPSDKLYVSYPIADAEGKTLLPSTVVKRLDELFPSSGEKVLVNEPEQLDDEEQLEFLVNKS
TALSYLTGQLGIWARQYAISDVWWSTYNFLMDEPDRGFSKNILSSLFFRNKVHRLTERVSRDLYGENIQGSVSRMETYKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKEQCDTLSSDAVERLAPRLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGLELGFGGNGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITYSAEWLGMKASPAGVLYFHVHDPMIQASVPLSLDEIEKEIFKKFKMKGL
LLGDREAIKLMDTALEQGRSDIISAGLKKDGSLRSDSDVVTEDDFHLLRRHVRRTFQKAGEEITDGNVSIEPYKLKDRTP
CTYCAFKSFCQFDESLEENEYRVLKPEKDNVILEWLKKEDEADGNG

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=336510 EQZ20_RS06250 WP_046131292.1 1240549..1244049(+) (addB) [Bacillus glycinifermentans strain SRCM103574]
ATGGGAGCAGTCTTTTTATCAGGCAGGTCCGGGAGTGGGAAAACGACCTTCATCTTGGATGACATCAGAGAAAAGCTTCG
CCATGACCCGCTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAGATGACGTTTTTAATGGAATATGAGCTTAGTAAAA
CTCCCGATTTGGGCGGGACGATCCGCGCCCAGGTTTACAGTTTTTCAAGGCTTGCCTGGCGAATCCTCCAGCATACGGGA
GGGATGAATCGTCCGTTTCTGACGGGGACGGGCATTCAAATGCTTCTTCGCAAATTGATCGAAGAGCATAAGAGCGAGTT
TAAAGTTTACCAGAATGCAAGCGATAAAAGCGGATTTACGGAGCAGGTCGAACGGATGCTGACCGAGTTTAAACGGCACT
GCCTGCCGCCTGAGGGCATTCGCGAGATCATGCAGGGAAGCGGGGCATCGGAATATGAGGAGGAACGGATTCTTTCCGAC
AAGCTCCATGACCTCTACATTTTATACAGCAGGATGGAAGAAGCTCTCGAGCATCAATACGTTCAGTCAGAAGATTATTT
GACGCTTTTGGCGGAGCAGATTCCGCACGCCGCAGAGATCAGGGACGCTTCGATTTATGTGGACGGTTTTCATCAATTCA
CCCCGCAGGAGATGCGCGTTCTTGAACAGCTGATGGCGCATGCCCGGGAGATTACGTTTTCTTTGACTGTGGACGAGCCT
TTCGCCCACCATGCACCGCATGATCTGCATCTGTTCCGGATGACGGGAAAGGCGTATTTCGATCTTTGCCAAAAGGCGCG
GGAGCTCGGCCTCGACTTGTCAGAGGTCCGGATGGAAGGGGCGAAACGGCACAGCCATGCCCCTGAACTTGAACACTTGG
AACGGCAGTTTGATGAACGGCCGGCAAAACCGTATACGGGAACATCGGAGTCTTTTTGCCTCATGCAGGCGGCAAACCGG
AGAACCGAAATCGAAGGGATCGCGAGGGAAATTCATTCCCTGGTGCATAGCGGCAGATTCAGGCTGAAAGATATCGCGGT
CATCGCCCGCAATCTCGAAGATTATAAAGATACGATAAAAGAAGTGTTTACGGATTACGAACTTTCTTTTTTCATAGACG
GAAAAGAGTCCATGCAGAATCATCCGCTGATCGAACTGATCCGTTCAACCCTTGATATCATCAAAAGCAACTGGCGATAT
GAAGCGGTGTTCCGCTGCATCAAAACAGAGCTTTTATTCCCGATGGATGAGCCGAGGGAGCGGGTGCGGGATCAGATCGA
CCAGCTCGAAAACTATTGCATTGCCTATGGGATTAAAGGCGAGAGATGGACGAGCAAAGACCCGTTTACATACCGCAGAT
ACGCCTCATTGGATGACGACTTTGCCAAGACCGACCGGGAGATTGAGACAGAAAATATGCTCAATGACTTAAGGAGCTGG
ATCGTACCGCCAGTCCACCGGCTGCAAAAACGCTTGAAAAAAGCGGGATCAGTCAGGGAAATGGCTGAAGCCGTTTATCT
TTATCTGGAAGAAGCGGACGTTCCGATGAAGCTGGAACAGGAACGGCAGCTTGCCGAAGAAAGCGGCCGGATCGCGGAAT
CAAGACAGCATGATCAAGTATGGGACGCAGTTATTCAATTGCTGGACGAGTTTGTGGAAATGATGGGAGACGAGCGCATA
TCGTTTTCCTTGTTTCAGCAAATGGTCGAAACCGGTTTGGAGTCTTTAACATTCTCGCTGATTCCGCCCGCTCTTGATCA
AGTATTTATCGGGAATATGGATTTATCTAGAATGTACGGGACGAAATGCACGTTCCTCATCGGGGTGAACGACGGCATTC
TTCCCGCAAGGCCGGCAGATGACGGGGTTTTGACAGATGACGACAGAGAGTGGCTGAGACGAAACGGTGTCCAGCTTGCA
GCAACGGGCAGAGAGCAGCTGCTTGACGAGCATTTTCTGATCTATATGGCGCTCTCAAGCCCGTCAGACAAGCTTTATGT
TTCATATCCGATCGCGGATGCCGAAGGAAAGACGCTGCTTCCTTCAACTGTTGTGAAACGATTGGACGAGCTGTTTCCAA
GTAGCGGAGAAAAGGTGCTTGTCAATGAACCGGAACAGCTTGACGATGAAGAACAGCTCGAATTTCTCGTCAATAAAAGC
ACGGCGTTAAGCTATTTGACAGGACAGCTTGGTATATGGGCGAGGCAGTATGCAATCAGTGATGTCTGGTGGAGCACATA
CAACTTTTTGATGGATGAGCCGGACCGCGGCTTTTCGAAAAATATTCTGTCCAGCTTGTTTTTCCGGAACAAGGTGCACA
GGCTCACCGAGCGCGTGAGCCGGGATTTGTACGGAGAGAACATCCAGGGGAGCGTGTCGCGGATGGAGACGTATAAAGCC
TGTCCGTTCTCCCATTTTGCTTCCCACGGCTTAAAGCTGAAGGAACGCCAATTTTTCAAGCTTGAAGCGCCTGATATCGG
CCAGCTGTTTCATTCCGCGCTGAAGCTGATTTCAGACCGACTTCATGAACTGAAGCTTGATTGGCGGGATCTGACGAAGG
AGCAATGCGATACATTGTCGAGCGATGCCGTGGAGCGGCTTGCGCCAAGGCTGCAAAAAGAGATATTGCTCAGTTCGAAC
CGCCACCACTATGTCAAGCAAAAGCTGCAAAAAATCATTGCAAGGGTGTCCGGCATTTTAAGCGAGCATGCAAAAGCGAG
CGGATTTGCTCCCGTCGGGCTGGAGCTGGGATTCGGCGGCAACGGACCGCTTCCTCCGATGCGGTTCACCCTTAAGAACG
GGTGCACAATGGAGCTTGTCGGCCGGATTGACAGGGTCGATAAAGCCGAAAGCTCAAAAGGTCTGCTGCTTCGAATCGTC
GATTACAAATCGAGCGATAAAGGCCTCGACTTAGCCGAAGTGTACTACGGCCTAGCCCTGCAGATGCTGACATACCTCGA
TTTGTCGATCACGTATTCGGCCGAATGGCTCGGGATGAAGGCATCGCCGGCCGGGGTTCTTTATTTTCACGTGCACGACC
CGATGATTCAGGCCTCCGTGCCGCTCAGCCTTGATGAGATCGAAAAGGAAATATTCAAGAAGTTTAAGATGAAAGGGCTC
CTGTTAGGGGACCGGGAAGCGATCAAGCTGATGGACACCGCCCTTGAACAGGGCAGGTCCGACATCATCAGCGCCGGCTT
GAAGAAGGACGGTTCTCTGCGTTCGGATTCGGATGTTGTCACGGAAGATGACTTTCATTTGTTAAGGCGCCACGTCCGCC
GGACGTTTCAAAAAGCAGGCGAGGAGATTACAGACGGCAATGTGTCGATCGAGCCTTATAAATTAAAGGACAGAACCCCT
TGCACATACTGCGCTTTCAAATCTTTCTGCCAGTTTGACGAATCGCTTGAAGAGAATGAATATCGCGTATTGAAGCCGGA
AAAAGACAATGTCATATTGGAATGGCTGAAAAAGGAGGATGAGGCAGATGGAAACGGCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

74.335

99.914

0.743

Multiple sequence alignment