Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FZC46_RS18940 Genome accession   NZ_CP043334
Coordinates   3900901..3901341 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain WCHEC115106     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3895901..3906341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZC46_RS18920 (FZC46_19015) aroP 3896899..3898272 (+) 1374 WP_001347572.1 aromatic amino acid transporter AroP -
  FZC46_RS18925 (FZC46_19020) ampE 3898315..3899169 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  FZC46_RS18930 (FZC46_19025) ampD 3899166..3899717 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FZC46_RS18935 (FZC46_19030) nadC 3899805..3900698 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FZC46_RS18940 (FZC46_19035) pilA 3900901..3901341 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FZC46_RS18945 (FZC46_19040) pilB 3901351..3902736 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  FZC46_RS18950 (FZC46_19045) hofC 3902726..3903928 (+) 1203 WP_000157236.1 protein transport protein HofC -
  FZC46_RS18955 (FZC46_19050) guaC 3903963..3905006 (-) 1044 WP_001217338.1 GMP reductase -
  FZC46_RS18960 - 3905162..3905206 (-) 45 WP_120795372.1 protein YacM -
  FZC46_RS18965 (FZC46_19055) coaE 3905231..3905851 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=336501 FZC46_RS18940 WP_000360895.1 3900901..3901341(+) (pilA) [Escherichia coli strain WCHEC115106]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=336501 FZC46_RS18940 WP_000360895.1 3900901..3901341(+) (pilA) [Escherichia coli strain WCHEC115106]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment