Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EQW70_RS01980 Genome accession   NZ_CP035226
Coordinates   362200..362649 (-) Length   149 a.a.
NCBI ID   WP_128740012.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103517     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 357200..367649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQW70_RS01940 (EQW70_01940) - 357519..357716 (+) 198 WP_122060560.1 hypothetical protein -
  EQW70_RS01945 (EQW70_01945) yckC 357736..358191 (+) 456 WP_076458396.1 RDD family protein -
  EQW70_RS01950 (EQW70_01950) yckD 358274..358606 (+) 333 WP_128740010.1 YckD family protein -
  EQW70_RS01955 (EQW70_01955) - 358735..359157 (+) 423 WP_170939093.1 MarR family transcriptional regulator -
  EQW70_RS01960 (EQW70_01960) - 359145..359486 (+) 342 WP_032724911.1 DUF3147 family protein -
  EQW70_RS01965 (EQW70_01965) - 359505..360233 (+) 729 WP_021481826.1 AAA family ATPase -
  EQW70_RS01970 (EQW70_01970) bglC 360305..361738 (+) 1434 WP_021481825.1 6-phospho-beta-glucosidase -
  EQW70_RS01975 (EQW70_01975) nin/comJ 361775..362173 (-) 399 WP_128740011.1 DNA-entry nuclease inhibitor Regulator
  EQW70_RS01980 (EQW70_01980) nucA/comI 362200..362649 (-) 450 WP_128740012.1 DNA-entry nuclease Machinery gene
  EQW70_RS01985 (EQW70_01985) tlpC 362817..364538 (-) 1722 WP_128740013.1 methyl-accepting chemotaxis protein TlpC -
  EQW70_RS01990 (EQW70_01990) hxlB 364648..365205 (-) 558 WP_021481820.1 6-phospho-3-hexuloisomerase -
  EQW70_RS01995 (EQW70_01995) hxlA 365211..365843 (-) 633 WP_032724921.1 3-hexulose-6-phosphate synthase -
  EQW70_RS02000 (EQW70_02000) hxlR 366075..366437 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16484.45 Da        Isoelectric Point: 4.5282

>NTDB_id=336187 EQW70_RS01980 WP_128740012.1 362200..362649(-) (nucA/comI) [Bacillus subtilis strain SRCM103517]
MNITTDIIKTILLVIVIIAAAAIGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDSTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=336187 EQW70_RS01980 WP_128740012.1 362200..362649(-) (nucA/comI) [Bacillus subtilis strain SRCM103517]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTATCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGAT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACAGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.315

100

0.973

Multiple sequence alignment