Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYK54_RS08160 Genome accession   NZ_CP043295
Coordinates   1690102..1690542 (+) Length   146 a.a.
NCBI ID   WP_020233150.1    Uniprot ID   -
Organism   Escherichia coli strain WCHEC040047     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1685102..1695542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK54_RS08140 (FYK54_08190) aroP 1686100..1687473 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  FYK54_RS08145 (FYK54_08195) ampE 1687516..1688370 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  FYK54_RS08150 (FYK54_08200) ampD 1688367..1688918 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYK54_RS08155 (FYK54_08205) nadC 1689006..1689899 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYK54_RS08160 (FYK54_08210) pilA 1690102..1690542 (+) 441 WP_020233150.1 prepilin peptidase-dependent pilin Machinery gene
  FYK54_RS08165 (FYK54_08215) pilB 1690552..1691937 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  FYK54_RS08170 (FYK54_08220) hofC 1691927..1693129 (+) 1203 WP_021522995.1 protein transport protein HofC -
  FYK54_RS08175 (FYK54_08225) guaC 1693164..1694207 (-) 1044 WP_001217337.1 GMP reductase -
  FYK54_RS08180 - 1694363..1694407 (-) 45 WP_120795372.1 protein YacM -
  FYK54_RS08185 (FYK54_08230) coaE 1694432..1695052 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=336186 FYK54_RS08160 WP_020233150.1 1690102..1690542(+) (pilA) [Escherichia coli strain WCHEC040047]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTNMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=336186 FYK54_RS08160 WP_020233150.1 1690102..1690542(+) (pilA) [Escherichia coli strain WCHEC040047]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCAACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACTACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment