Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KOCBH_RS21515 Genome accession   NZ_CP035214
Coordinates   4463337..4464179 (+) Length   280 a.a.
NCBI ID   WP_119834446.1    Uniprot ID   A0AB35Q1H5
Organism   Klebsiella michiganensis strain M82255     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4446660..4463275 4463337..4464179 flank 62


Gene organization within MGE regions


Location: 4446660..4464179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOCBH_RS33790 (KOCBH_04186) - 4446660..4447091 (+) 432 WP_014228288.1 mannose/fructose/sorbose PTS transporter subunit IIA -
  KOCBH_RS33795 (KOCBH_04187) - 4447093..4447569 (+) 477 WP_014228289.1 PTS system mannose/fructose/N-acetylgalactosamine-transporter subunit IIB -
  KOCBH_RS21440 (KOCBH_04188) - 4447581..4448381 (+) 801 WP_014228290.1 PTS mannose/fructose/sorbose transporter subunit IIC -
  KOCBH_RS21445 (KOCBH_04189) - 4448393..4449241 (+) 849 WP_014228291.1 mannose/fructose/sorbose PTS transporter subunit IID -
  KOCBH_RS33550 (KOCBH_04190) - 4449569..4452061 (+) 2493 WP_407057977.1 GH32 C-terminal domain-containing protein -
  KOCBH_RS21455 (KOCBH_04191) gspC 4452342..4453199 (+) 858 WP_128696797.1 type II secretion system protein GspC -
  KOCBH_RS21460 (KOCBH_04192) gspD 4453215..4455179 (+) 1965 WP_407057990.1 type II secretion system secretin GspD -
  KOCBH_RS21465 (KOCBH_04193) gspE 4455179..4456684 (+) 1506 WP_014228296.1 type II secretion system ATPase GspE -
  KOCBH_RS21470 (KOCBH_04194) gspF 4456689..4457909 (+) 1221 WP_128696799.1 type II secretion system inner membrane protein GspF -
  KOCBH_RS21475 (KOCBH_04195) gspG 4457939..4458367 (+) 429 WP_042944533.1 type II secretion system major pseudopilin GspG -
  KOCBH_RS21480 (KOCBH_04196) gspH 4458367..4458918 (+) 552 WP_119834450.1 type II secretion system minor pseudopilin GspH -
  KOCBH_RS21485 (KOCBH_04197) gspI 4458915..4459280 (+) 366 WP_119834449.1 type II secretion system minor pseudopilin GspI -
  KOCBH_RS21490 (KOCBH_04198) gspJ 4459277..4459876 (+) 600 WP_014228301.1 type II secretion system minor pseudopilin GspJ -
  KOCBH_RS21495 (KOCBH_04199) gspK 4459873..4460859 (+) 987 WP_042944535.1 type II secretion system minor pseudopilin GspK -
  KOCBH_RS21500 (KOCBH_04200) gspL 4460859..4462064 (+) 1206 WP_128696800.1 type II secretion system protein GspL -
  KOCBH_RS21505 (KOCBH_04201) gspM 4462051..4462545 (+) 495 WP_014228304.1 type II secretion system protein GspM -
  KOCBH_RS21510 (KOCBH_04202) - 4462535..4463275 (+) 741 WP_128696801.1 type II secretion system protein N -
  KOCBH_RS21515 (KOCBH_04203) pilD 4463337..4464179 (+) 843 WP_119834446.1 A24 family peptidase Machinery gene

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 30587.69 Da        Isoelectric Point: 8.8641

>NTDB_id=336081 KOCBH_RS21515 WP_119834446.1 4463337..4464179(+) (pilD) [Klebsiella michiganensis strain M82255]
MTLLVAFSSTYPIIWWFGLGATGAIVGSFLNVVICRLPVMLEKHWQREALLQLSLPSPEPTARFNLVLPHSRCPYCHTPV
AARDNIPLLSFLLLKGKTRCCGAPISAQYPLVEAATAALFVLTARVFPPGLALCGAWVFVSFLLILAVIDYHRQLLPDLL
TLPLLWIGLLFNLQSYFVSLPQATIGAVAGYLCLWCLFWLFKLLTGKDALGYGDFKLLAALGAWLGWQALPHVVLLAALS
GLLFAFVQRGGAKKDLNQPLAFGPWLALAGVANMLLFQGI

Nucleotide


Download         Length: 843 bp        

>NTDB_id=336081 KOCBH_RS21515 WP_119834446.1 4463337..4464179(+) (pilD) [Klebsiella michiganensis strain M82255]
ATGACATTATTAGTGGCTTTTTCATCCACGTATCCCATCATTTGGTGGTTTGGTCTTGGGGCGACAGGCGCTATCGTCGG
GAGTTTTCTGAATGTGGTGATCTGCCGCTTACCCGTAATGCTCGAGAAACACTGGCAGCGTGAGGCGTTGCTGCAGCTGT
CACTGCCTTCACCTGAACCCACGGCGCGCTTTAATCTGGTATTGCCGCACTCTCGTTGCCCATATTGTCATACTCCTGTT
GCCGCAAGGGATAATATACCTCTGCTGAGTTTCCTGTTGTTAAAGGGCAAGACTCGCTGTTGCGGCGCGCCTATTTCCGC
GCAGTATCCGCTGGTTGAGGCGGCCACCGCTGCGCTGTTTGTCTTAACCGCACGTGTATTTCCGCCGGGGCTGGCGCTGT
GTGGCGCCTGGGTATTCGTCTCTTTTTTATTGATCCTGGCCGTTATTGATTATCACCGACAGCTGCTACCCGATCTCCTG
ACGCTGCCCTTATTGTGGATCGGCTTACTGTTTAATCTTCAGTCGTATTTTGTTTCGTTGCCGCAGGCGACGATTGGCGC
AGTGGCAGGATATCTCTGCTTGTGGTGCCTGTTCTGGCTGTTCAAGCTACTGACAGGCAAGGATGCGCTGGGCTATGGCG
ATTTTAAGCTACTGGCCGCGTTGGGAGCCTGGCTAGGCTGGCAAGCATTACCCCATGTGGTTTTACTGGCGGCGTTAAGC
GGATTGCTGTTTGCGTTCGTACAGCGAGGAGGGGCAAAAAAAGATCTCAATCAGCCGCTGGCATTCGGCCCGTGGCTGGC
GTTAGCCGGTGTGGCAAATATGCTGCTATTTCAGGGTATTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.06

95.357

0.496
  pilD Vibrio campbellii strain DS40M4

49.077

96.786

0.475
  pilD Neisseria gonorrhoeae MS11

48.193

88.929

0.429
  pilD Acinetobacter nosocomialis M2

43.902

87.857

0.386
  pilD Acinetobacter baumannii D1279779

43.496

87.857

0.382

Multiple sequence alignment