Detailed information NTG0336078

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: KOCBH_RS19095
• Genome accession: NZ_CP035214
• Coordinates: 3946556..3947353 (-)
• Length: 265 a.a.
• NCBI ID: WP_014227970.1
• Uniprot ID: A0A0H3H8P5
• Organism: Klebsiella michiganensis strain M82255
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3941556..3952353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KOCBH_RS19075 (KOCBH_03721)	dksA	3941820..3942275 (-)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-
KOCBH_RS19080 (KOCBH_03722)	sfsA	3942440..3943156 (-)	717	WP_025106516.1	DNA/RNA nuclease SfsA	-
KOCBH_RS19085 (KOCBH_03723)	thpR	3943156..3943692 (-)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
KOCBH_RS19090 (KOCBH_03724)	hrpB	3943765..3946194 (+)	2430	WP_049102372.1	ATP-dependent helicase HrpB	-
KOCBH_RS19095 (KOCBH_03725)	pilD	3946556..3947353 (-)	798	WP_014227970.1	A24 family peptidase	Machinery gene
KOCBH_RS19100 (KOCBH_03726)	-	3947356..3948114 (-)	759	WP_064405742.1	type II secretion system protein N	-
KOCBH_RS19105 (KOCBH_03727)	gspM	3948104..3948589 (-)	486	WP_128696715.1	type II secretion system protein GspM	-
KOCBH_RS19110 (KOCBH_03728)	gspL	3948582..3949775 (-)	1194	WP_025107363.1	type II secretion system protein GspL	-
KOCBH_RS19115 (KOCBH_03729)	gspK	3949768..3950748 (-)	981	WP_048262360.1	type II secretion system minor pseudopilin GspK	-
KOCBH_RS19120 (KOCBH_03730)	gspJ	3950745..3951341 (-)	597	WP_048262357.1	type II secretion system minor pseudopilin GspJ	-
KOCBH_RS19125 (KOCBH_03731)	gspI	3951338..3951703 (-)	366	WP_048262356.1	type II secretion system minor pseudopilin GspI	-
KOCBH_RS19130 (KOCBH_03732)	gspH	3951700..3952212 (-)	513	WP_048262354.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29166.77 Da
• Isoelectric Point: 8.3358

>NTDB_id=336078 KOCBH_RS19095 WP_014227970.1 3946556..3947353(-) (pilD) [Klebsiella michiganensis strain M82255]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=336078 KOCBH_RS19095 WP_014227970.1 3946556..3947353(-) (pilD) [Klebsiella michiganensis strain M82255]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0H3H8P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434